利用基因编辑技术创制福香占糯稻新种质

Creation of Novel Glutinous Rice Germplasm of Fuxiangzhan(FXZ) Using Genome Editing

福香占是新育成的优质、抗稻瘟病、长粒籼稻品种,具有较好的推广潜力。本研究旨在利用基因编辑技术快速创制福香占糯稻材料。通过筛选和优化靶点序列,成功地利用CRISPR/Cas9技术敲除水稻颗粒结合型淀粉合酶基因(Waxy,Wx),获得了Wx基因的缺失突变体。通过后代农艺性状和米质鉴定,结果表明敲除材料的淀粉含量从16.3%下降至1.67%~1.81%,达到了糯稻水平。此外,敲除植株在株叶态、产量和抗性方面并没有显著变化。此项研究为福香占的应用推广提供了新的途径,并丰富了抗瘟糯稻的种质资源。

Gene editing is an emerging genetic improvement technology that allows for rapid and precise modification of crop traits.FXZ is a newly developed high-quality,blast-resistant,long-grain indica rice variety with great potential for commercialization.This study aimed to utilize gene editing technology to create FXZ glutinous rice materials rapidly.By screening and optimizing target sequences,the rice granule-bound starch synthase gene(Waxy,Wx)was successfully knocked out using CRISPR/Cas9 technology,resulting in Wx gene knockout mutants.Through progeny agronomic traits evaluation and amylose content determination,the results showed that the amylose content of the knockout materials decreased from 16.3%to approximately 1.67%-1.81%,meeting the criteriaof glutinous rice.Additionally,there were no significant changes observed in plant morphology,yield,and resistance in the mutant plants.This study provides a new approach for the application and promotion of FXZ and enriches the genetic resources of blast-resistant glutinous rice.