血清miR-140-5p、PGC-1αmRNA表达与急性缺血性脑卒中患者血管性认知障碍的相关性

Relation of serum miR-140-5p and PGC-1αmRNA expression with vascular cognitive impairment in acute ischemic stroke patients

目的探讨血清miR-140-5p、过氧化物酶体增殖物激活受体γ共激活剂1α(PGC-1α)mRNA表达与急性缺血性脑卒中(AIS)患者血管性认知障碍(VCI)的关系。方法选取2020年1月—2022年1月石家庄市人民医院AIS患者163例(AIS组)和体检健康者57名(正常对照组),收集所有研究对象一般资料。根据3个月后是否并发VCI将AIS患者分为VCI组和非VCI组。检测所有研究对象血清miR-140-5p和PGC-1αmRNA相对表达量。采用Logistic回归分析评估AIS患者发生VCI的危险因素。采用受试者工作特征(ROC)曲线评价血清miR-140-5p、PGC-1αmRNA判断AIS患者发生VCI的效能。结果AIS组血清miR-140-5p相对表达量高于正常对照组(P<0.001),PGC-1αmRNA相对表达量低于正常对照组(P<0.001)。VCI组血清miR-140-5p相对表达量高于非VCI组(P<0.05),PGC-1αmRNA相对表达量低于非VCI组(P<0.05)。多因素Logistic回归分析结果显示,美国国立卫生研究院卒中量表(NIHSS)评分、血清miR-140-5p相对表达量升高和PGC-1α相对表达量降低是AIS患者发生VCI的独立危险因素[比值比(OR)值分别为3.298、1.438、0.716,95%可信区间(CI)分别为1.983~5.485、1.147~1.801、0.585~0.876]。ROC曲线分析结果显示,miR-140-5p、PGC-1αmRNA单项检测和联合检测判断AIS患者发生VCI的曲线下面积(AUC)分别为0.790、0.780、0.905。结论血清miR-140-5p表达升高和PGC-1αmRNA表达降低与AIS患者VCI的发生密切相关,或可作为AIS患者发生VCI的预测指标。

Objective To investigate the relation between serum miR-140-5p and peroxisome-proliferatoractivated receptor gamma coactivator-1 alpha(PGC-1α)mRNA expression and vascular cognitive impairment(VCI)in acute ischemic stroke(AIS)patients.Methods Totally,163 patients with AIS in Shijiazhuang People's Hospital from January 2020 to January 2022(AIS group)were enrolled and classified into VCI group and non-VCI group according to whether they had VCI after 3 months or not.Totally,57 healthy subjects were enrolled as control group.The general data of all the subjects were collected,and serum miR-140-5p and PGC-1αmRNA expression were determined.Logistic regression analysis was used to analyze the factors on VCI in AIS patients,and receiver operating characteristic(ROC)curve was used to analyze the predictive value of serum miR-140-5p and PGC-1αmRNA expression on VCI in AIS patients.Results Serum miR-140-5p expression in AIS group was higher than that in control group(P<0.001),and PGC-1αmRNA expression was lower than that in control group(P<0.001).The miR-140-5p expression was higher in VCI group than that in non-VCI group(P<0.05),and PGC-1αmRNA expression was lower than that in non-VCI group(P<0.05).Increased the National Institute of Health Stroke Scale(NIHSS)score[odds ratio(OR)=3.298,95%confidence interval(CI)1.983-5.485],increased miR-140-5p expression(OR=1.438,95%CI 1.147-1.801)and decreased PGC-1αmRNA expression(OR=0.716,95%CI 0.585-0.876)were risk factors for VCI in AIS patients.The areas under curves(AUC)of miR-140-5p and PGC-1αmRNA expression single and combined determinations for VCI in AIS patients were 0.790,0.780 and 0.905,respectively.Conclusions Increased serum miR-140-5p expression and decreased PGC-1αmRNAexpression are related with VCI in AIS patients and may be predictors of VCI in AIS patients.