磷转运蛋白StPHO1.2提高马铃薯耐热性

Phosphorus transporter StPHO1.2 improving heat tolerance in potato

植物通过磷转运蛋白吸收和转运磷元素,充足的磷元素能够提高作物产量、品质和抗逆性。高温是影响马铃薯生长发育的重要环境因子,严重时会造成减产甚至绝收。本研究利用农杆菌介导,在马铃薯(Solanum tuberosum L.)中过表达磷转运蛋白基因StPHO1.2,比较转基因株系和野生型株系在高温(35℃)和常温(22℃±1℃)环境下的生长状况。结果表明,过表达StPHO1.2能够提高马铃薯耐热性,促进其生长,且磷元素浓度越高,抗性越强,长势越好。本氏烟草中的亚细胞定位结果显示,StPHO1.2在细胞膜上表达,因此,选择膜系统文库质粒筛选StPHO1.2的互作蛋白。通过酵母双杂交试验和BiFC试验,本研究证明磷转运蛋白StPHO1.2与钙离子转运相关蛋白(StCAX1)和光系统Ⅱ蛋白亚基(StPsbR)均有相互作用。综上所述,过表达StPHO1.2可能通过影响光合作用和信号转导,从而提高马铃薯耐热性,促进马铃薯生长。这些结果为深入理解磷转运蛋白的功能提供了理论依据和参考,对马铃薯新品种的选育具有促进作用。

Plants absorb and transport phosphorus through phosphorus transporters,and sufficient phosphorus can improve crop yield,quality,and stress resistance.High temperature is an important environmental factor affecting the growth and development of potato.In this study,we overexpressed the phosphorus transporter StPHO1.2 in potato mediated by Agrobacterium tumefaciens,and then compared the growth of transgenic strains and wild-type strains at high temperature(35℃)and normal temperature(22℃±1℃).The results showed that overexpression of StPHO1.2 could improve the heat resistance and promote the growth of potato.And the higher the phosphorus concentration,the stronger the resistance,the better the growth.Subcellular localization in tobacco showed that StPHO1.2 was expressed on cell membrane.Therefore,the membrane system library plasmid was selected to screen the interacting proteins of StPHO1.2.This study demonstrated that the phosphorus transporter StPHO1.2 interacted with both the calcium ion transporter associated protein(StCAX1)and the photosynthetic system II protein subunit(StPsbR)by the yeast two-hybrid and BiFC.In conclusion,overexpression of StPHO1.2 may improve the heat tolerance and promote the growth of potato by affecting photosynthesis and signal transduction in potato.These results provide theoretical basis and reference for further understanding of the function of phosphorus transporters and promote the breeding of new potato varieties.