粪肠球菌EF-ZA1107-06的体外益生特性研究

Study on Beneficial Characteristics in Vitro of Enterococcus Faecalis EF-ZA1107-06

已有研究表明,益生菌具有良好的抗糖尿病和抗氧化活性,并对人类癌细胞系有很强的抑制作用。本研究以α-葡萄糖苷酶和α-淀粉酶抑制率、超氧阴离子自由基清除率、亚铁离子螯合率和还原活性为指标,比较了粪肠球菌(Enterococcus faecalis)EFZA1107-06和鼠李糖乳杆菌(Lactobacillus.rhamnosus GG,LGG)的体外抗糖尿病和抗氧化能力;通过测量Caco-2细胞系中谷胱甘肽过氧化物酶(GSH-Px)、总超氧化物歧化酶(TSOD)和过氧化氢酶(CAT)的活性,研究了EF-ZA1107-06对细胞氧化应激的影响,并用HepG2和MDA-MB-231细胞系初步探讨了其抗癌活性和机制。结果表明,EF-ZA1107-06的上清液对超氧阴离子自由基的清除率最高(40.78%~59.61%),对亚铁离子的螯合能力最强(39.28%~56.59%),而EF-ZA1107-06裂解液的还原活性最高,相当于1.072 mmol/L当量半胱氨酸,明显高于LGG裂解液(P<0.05)。对α-淀粉酶和α-葡萄糖苷酶活性的抑制证实了EF-ZA1107-06具有抗糖尿病活性;同时,EF-ZA1107-06能在一定程度上减少H2O2对Caco-2细胞造成的氧化损伤,抑制HepG2细胞的S期和G2期,但只抑制MDAMB-231细胞的S期;诱导HepG2细胞和MDA-MB-231细胞早期凋亡的结果证实了EF-ZA1107-06具有抗癌活性。

Studies have shown that probiotic has good antidiabetic and antioxidant activities and great inhibitory effects on human cancer cell lines.This paper tookα-glucosidase andα-amylase inhibition rate,superoxide anion radical scavenging rate,ferrous ion chelating rate and reducing activity as indexes,and compared the antidiabetic and antioxidant capacities in vitro between Enterococcus faecalis EF-ZA1107-06 and Lactobacillus rhamnosus GG(LGG).The effects of EF-ZA1107-06 on cellular oxidative stress were investigated by measuring the enzyme activities of glutathione peroxidase(GSH-Px),total superoxide dismutase(T-SOD)and catalase(CAT)in Caco-2 cell lines.The anticancer activity and mechanism were also preliminarily explored using HepG2 and MDA-MB-231 cell lines.The results show that the supernatant of EF-ZA1107-06 has the highest scavenging rate of superoxide anion free radical from 40.78%to 59.61%and strongest ferrous ion chelating ability from 39.28%to 56.59%,while the lysate of EF-ZA1107-06 has the highest reducing activity,equivalent to 1.072 mmol/L equivalent cysteine,which is remarkably higher than that of LGG lysate(P<0.05).The inhibition ofα-amylase andα-glucosidase activity confirms the antidiabetic activity of EF-ZA1107-06.Meanwhile,EF-ZA1107-06 can reduce oxidative damage caused by H2O2 to Caco-2 cells to a certain extent and inhibit the S phase and G2 phase of HepG2 cells,but only the S phase of MDA-MB-231 cells.The induction of the early apoptosis of HepG2 cells and MDA-MB-231 cells confirms the anticancer activity of EF-ZA1107-06.