酸枣组培快繁体系的建立

The establishment of tissue culture and rapid propagation system of sour jujube

酸枣对环境的适应能力很强,耐干旱、瘠薄,且具有较高的经济价值。愈伤组织是进行植物离体培养的常用材料,但是酸枣组培体系的建立相关研究报道较少。选取了20个基因型酸枣,探究其组织培养过程中外植体、激素配比对不同基因型酸枣愈伤组织诱导、愈伤组织芽诱导以及生根诱导的影响。结果表明:酸枣组培快繁体系中愈伤组织的最佳诱导培养基配方为MS+3.0 mg/L TDZ+0.5 mg/L NAA;酸枣愈伤组织芽再生最佳诱导培养基配方为MS+1.5 mg/L 6-BA+0.5 mg/L IBA、MS+2.0 mg/L 6-BA+0.2 mg/L IBA;酸枣芽的根再生最佳诱导培养基配方为1/2 MS+1.0 mg/L IBA、1/2 MS+1.0 mg/L IBA+0.4 mg/L NAA。建立了适宜大部分酸枣的组培快繁体系,可为将来大规模的酸枣组培扩繁提供重要的理论指导。

Sour jujube has strong adaptability to the environment,including drought and barrenness resistance,with high economic value.Callus is a common material for ex-vivo cultivation,but few reports on the establishment of sour jujube tissue cultivating system were shown.In the current study,twenty sour jujubes were selected to explore the effect of genotypes,explants and hormone ratios on the induction of callus,bud and root.The results showed that the optimal callus inducing medium in sour jujube fast propagation system was MS+3.0 mg/L TDZ+0.5 mg/L NAA.The optimal bud inducing medium of sour jujube callus was MS+1.5 mg/L 6-BA+0.5 mg/L IBA,MS+2.0 mg/L 6-BA+0.2 mg/L IBA.The optimal root inducing medium of sour jujube was 1/2 MS+1.0 mg/L IBA and 1/2 MS+1.0 mg/L IBA+0.4 mg/L NAA.The rapid tissue cultivating system suitable for most sour jujubes was established,which could provide important theoretical guidance for large-scale tissue cultivation of sour jujube in the future.