胶原蛋白肽部分恢复辐射联合模拟失重模型小鼠的免疫功能

Collagen peptide partially restores immune function in mice under the condition of X-ray irradiation combined with simulated weightlessness

目的探讨胶原蛋白肽对X线辐射联合模拟失重小鼠免疫功能的影响。方法小鼠分为对照组、模型组和胶原蛋白肽组。从实验的第1天开始,模型组小鼠每千克体质量每天1次腹腔注射600 mg的胶原蛋白肽,其余2组小鼠腹腔注射生理盐水。于实验的第4天模型组和胶原蛋白肽组小鼠进行X线辐照和尾吊,于实验的第10天断颈处死小鼠,采用全自动血细胞分析仪检测外周血白细胞分类,采用流式细胞术检测脾脏淋巴细胞亚群及骨髓细胞的细胞周期和凋亡情况,采用液态悬浮芯片法检测血浆19种细胞因子。结果与对照组相比,模型组小鼠外周血白细胞总数和淋巴细胞数,脾细胞总数和脾脏中T细胞、CD4^(+)T细胞和CD8^(+)T细胞、B细胞、自然杀伤(NK)细胞数量均显著降低,18种细胞因子降低;骨髓细胞凋亡增加。与模型组小鼠相比,除某些细胞因子外,胶原蛋白肽组小鼠其余多数免疫学指标均有改善。结论胶原蛋白肽能够有效改善X线辐射联合模拟失重小鼠的免疫功能。

Objective To investigate the effects of collagen peptides on the immune function of mice under the condition of X-ray irradiation combined with simulated weightlessness.Methods Mice were randomly divided into control group,modelling group and collagen peptide group.Mice in collagen peptide group were intraperitoneally injected with collagen peptide(600 mg/kg)once a day from the first day of the experiment,while mice in the other two groups were intraperitoneally injected with normal saline.On the fourth day of the experiment,mice in the modelling group and collagen peptide group were simultaneously exposed to X-ray irradiation(2 Gy)and hindlimb-unloaded simulated weightlessness by tail-suspension.On the 10th day of the experiment,the mice were terminated by cervical dislocation.Automated hematology analyzer was used to detect Leukocyte classification of peripheral blood.Splenic lymphocyte subsets,cell cycle and apoptosis of bone marrow cells were analyzed by flow cytometry.The expressions of 19 plasma cytokines were tested with liquid suspension chips.Results Compared with the control group,the modelling group had a significant reduction in the total number of white blood cells and lymphocytes in the peripheral blood,the total number of splenocyte and the number of T cells,CD4^(+)and CD8^(+)T cells,B cells,and natural killer(NK)cells in the spleen,an decrease in 18 cytokines in the plasma,and an increase in myelocyte apoptosis in mice of the modelling group.Compared with the modelling group,most immunological parameters had improved in the mice of the collagen peptide group except some cytokines.Conclusion Collagen peptides can effectively improve the immune function of mice under the condition of X-ray irradiation combined with simulated weightlessness.