可用于人造血管内层基底的电纺细菌纤维素/聚氨酯复合膜的制备与表征

Manufacturing and representation of electrospun BC/PU composite membrane for inner substrate of artificial blood vessels

将聚氨酯与细菌纤维素结合制备复合材料是原位组织工程中探索可降解自愈型人造血管的一个颇有前景的方向。研究了一种制备细菌纤维素(BC)/聚氨酯(PU)复合膜的方法,并评判其用于人造血管内层基底的应用潜力。将细菌纤维素超细粉末与聚氨酯充分混合,利用静电纺丝的技术成功制备细菌纤维素/聚氨酯复合膜,采用傅里叶红外光谱分析仪、DSC差示扫描量热仪、3D电子显微镜、扫描电子显微镜,接触角测量仪及拉伸断裂强力仪等测试仪器对复合膜的外观形貌及物理化学性能进行测试后发现:制备的复合膜同时具有细菌纤维素和聚氨酯的物理化学特性,且具有良好的亲水性及纤维网格结构,有利于提高人造血管内层的生物相容性及抗血栓性相关的血液相容性。结果表明:静电纺丝液溶质质量分数(ω溶质)为29%,其中细菌纤维素质量分数[ω(BC)]为5%~9%,聚氨酯质量分数[ω(PU)]为20%~24%时,制备的复合膜具有较好的物理化学性能,具有作为人造血管内层基底的应用潜力。

The combination of polyurethane and bacterial cellulose to prepare composite materials is a promising direction for exploring biodegradable self-healing artificial blood vessels in in-situ tissue engineering.A method for preparing bacterial cellulose(BC)/polyurethane(PU)composite membranes was studied and its potential application as an inner layer substrate for artificial blood vessels was evaluated.The bacterial cellulose ultrafine powder was fully mixed with polyurethane,and the bacterial cellulose/polyurethane composite film was successfully prepared by using electrospinning technology.The appearance,morphology,and physicochemical properties of the composite film were tested by using testing instruments,such as Fourier transform infrared spectroscopy,DSC differential scanning calorimeter,3D electron microscope,scanning electron microscope,contact angle measuring instrument,and tensile fracture strength tester;And it came to that prepared composite membrane had both the physical and chemical properties of bacterial cellulose and polyurethane,the good hydrophilicity andfiber grid structure,which was conducive to improving the biocompatibility of the inner layer of artificial blood vessels and blood compatibility related to anti thrombotic properties.The results showed that when the solute mass fraction(ωsolute)of electrospinning solution was 29%,among which the mass fraction of bacterial cellulose[ω(BC)]was 5%9%,andthe mass fraction of polyurethane[ω(PU)]was between 20%and 24%,the prepared composite membrane had relatively good physical and chemical properties,as well as the potential to be used as the inner substrate of artificial blood vessels.