低离心力对正常及不液化精液精子形态分析结果的影响分析

Effects of low centrifugal force on sperm morphology of normal and non⁃liquefied semen samples

目的探讨低离心力对正常精液与不液化精液精子形态分析结果的影响。方法收集2021年1月至12月在解放军联勤保障部队第904医院生殖中心进行精液常规检查与精子形态学检查的169例患者作为研究对象,其中126例为精液常规检查正常患者,43例为精液不液化患者。比较126例正常精液采用直接涂片与两种不同低离心力下离心处理所得精子形态学分析结果差异;比较43例不液化精液采用促液化后直接涂片与离心处理所得精子形态学结果差异。结果正常精液标本340×g离心10 min所得的正常形态精子百分率[(9.39±0.50)%]较直接涂片法[(11.08±0.41)%]显著降低,所得精子中段异常率[(62.05±1.44)%]较直接涂片法[(57.17±0.82)%]显著增加;151×g离心10 min所得的正常形态精子百分率[(9.41±0.57)%]亦较直接涂片法显著降低,所得精子中段异常率[(60.95±1.17)%]亦较直接涂片法显著增加。对于不液化精液标本,340×g离心10 min所得的正常形态精子百分率[(9.61±0.60)%]与促液化后直接涂片法[(11.10±0.73)%]差异无统计学意义,所得精子中段异常率[(60.21±1.51)%]与促液化后直接涂片法[(57.36±1.34)%]差异无统计学意义。结论两种低离心力均对精子形态学造成影响。离心力对不液化精液无显著影响。检查精子形态学的临床应用中应参考男性精液性质决定是否离心以及离心力大小。

Objective To explore the effects of low centrifugal force on sperm morphology analysis of normal and nonliquefied semen samples.Methods A total of 169 patients who underwent semen routine examination and sperm morphology analysis at the Reproductive Center of the 904th Hospital of the Joint Logistics Support Force of PLA from January 2021 to December 2021 were collected as the study subjects.Among them,126 were the patients with normal semen routine parameters,and 43 with nonliquefied semen.The morphological analysis results of 126 normal semen samples treated by the direct smear method and centrifugation methods under two different of low centrifugal force were compared.The results of morphological analysis for the 43 nonliquefied semen samples treated by the direct smear method after promoting liquefaction and centrifugation were compared.Results The percentage of normal morphological sperm[(9.39±0.50)%]obtained by centrifugation of 340×g centrifugal force for 10 min was significantly lower than that[(11.08±0.41)%]obtained by the direct smear method,and the abnormal rate of sperm in the middle segment[(62.05±1.44)%]obtained by centrifugation of 340×g centrifugal force was significantly higher than that[(57.17±0.82)%]obtained by the direct smear method.The percentage of normal morphological sperm[(9.41±0.57)%]obtained by centrifugation at 151×g centrifugal force was significantly lower than that obtained by the direct smear method,and the abnormal rate of sperm in the middle segment[(60.95±1.17)%]obtained by centrifugation at 151×g centrifugal force was significantly higher than that obtained by the direct smear method.For nonliquefied semen samples,the percentage of normal morphological sperm obtained by centrifugation at 340×g centrifugal force for 10 min was[(9.61±0.60)%],while that obtained by the direct smear method after promoting liquefaction was[(11.10±0.73)%].The difference was not statistically significant between the two methods.The abnormal rate of sperm in the middle segment[(60.21±1.51)%]obtained by the centrifugation method was also not significantly different from that[(57.36±1.34)%]obtained by the direct smear method after promoting liquefaction.Conclusion Both kinds of low centrifugal force affected the sperm morphology.However,the low centrifugal force has no significant effect on the nonliquefied semen.In the clinical application of sperm morphology analysis,the nature of male semen should be referred to determine whether the centrifugation is need or not and the magnitude of centrifugal force.