摘要
目的探究Mertk表达水平对大鼠雪旺细胞NF-κb通路的调控作用及其可能的机制。方法体外培养大鼠雪旺细胞,免疫印迹法检测Mertk在高糖环境雪旺细胞中表达情况。免疫共沉淀法检测内源性Mertk与Ikbkb相互作用。免疫印迹法检测沉默Mertk后雪旺细胞内Ikbkb、P65、肿瘤坏死因子-α表达水平。免疫荧光法检测沉默Mertk后Mertk、Ikbkb、P65蛋白表达。结果Mertk在雪旺细胞中存在表达,且表达量随葡萄糖浓度增加而提高;免疫共沉淀实验表明Mertk与Ikbkb在大鼠雪旺细胞内相互作用;免疫印迹实验显示,将雪旺细胞内Mertk敲减表达后,与对照组相比,Mertk表达水平降低(P<0.05),Ikbkb、P65、TNF-α明显升高(P<0.05);免疫荧光实验显示,沉默后的雪旺细胞内Mertk荧光减弱,Ikbkb、P65荧光增强。结论Mertk表达降低后,可以通过与Ikbkb进行相互作用,介导调控雪旺细胞NF-κb通路,上调P65和炎症因子TNF-α的表达。
Objective To explore the regulatory effect of Mertk expression level on NF-κb pathway in rat Schwann cells and its possible mechanism.Methods Rat Schwann cells were cultured in vitro,and the expression of Mertk in Schwann cells exposed to high glucose was detected by Western blot.Co-immunoprecipitation was used to detect the interaction between endogenous Mertk and Ikbkb.Western blot was used to detect the expression levels of Ikbkb,P65 and tumor necrosis factor-αin Schwann cells after Mertk silencing.The protein expressions of Mertk,Ikbkb and P65 after silencing Mertk were detected by immunofluorescence.Results Mertk was expressed in Schwann cells,and the expression level increased with the increase of glucose concentration.Co-immunoprecipitation assay showed that Mertk interacted with Ikbkb in rat Schwann cells.Compared with the control group,the expression level of Mertk was significantly decreased(P<0.05),while Ikbkb,P65 and TNF-αwere significantly increased(P<0.05)after knock down expression of Mertk in Schwann cells.Immunofluorescence experiments showed that the fluorescence of Mertk was decreased,and the fluorescence of Ikbkb and P65 was increased in the silenced Schwann cells.Conclusion After the expression of Mertk is decreased,it can mediate the regulation of NF-κb pathway in Schwann cells through interaction with Ikbkb,and up-regulate the expression of P65 and inflammatory factor TNF-α.
作者
付怡丹
陈文婷
苏晓杨
赵燕
兰丹凤
杨秋萍
FU Yidan;CHEN Wenting;SU Xiaoyang;ZHAO Yan;LAN Danfeng;YANG Qiuping(Dept.of Geriatrics,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032;Dept.of Critical Care Medicine,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032;Dept.of Diabetes,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032;Dept.of Gastroenterology,The 1st People's Hospital of Yunnan Province,Kunming Yunnan 650032;Dept.of Endocrinology,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)
出处
《昆明医科大学学报》
CAS
2023年第12期20-24,共5页
Journal of Kunming Medical University
基金
云南省科技厅科技计划基金资助项目(202001AY070001-159)。