大花海棠‘比哥’离体快繁再生体系的初步建立

Preliminary Establishment of Tissue Culture Regeneration System for Begonia benariensis‘BIG’

大花海棠‘比哥’系列品种具有较高的观赏和经济价值,其开发前景广阔,但是中国种植的大花海棠‘比哥’系列品种依赖于国外进口。以MS和1/2MS为基本培养基,分别采用不同的植物激素组合,研究组合培养基对大花海棠不定芽分化、不定芽继代增殖和生根的影响,建立大花海棠组培快繁体系,以解决生产上面临的种质资源困境。大花海棠不定芽分化最佳培养基为MS+1 mg/L 6-BA+0.1 mg/L 2.4-D+0.2 mg/L NAA,不定芽继代增殖最佳培养基为MS+1 mg/L 6-BA+0.1 mg/L NAA,组培苗生根的最佳培养基为1/2MS+1.0 mg/L NAA。试验建立了一套大花海棠再生体系的完整方案,可为大花海棠的大规模培养、繁殖提供理论依据。

The Begonia benariensis‘BIG’has high ornamental and economic value,and has broad application prospects.However,the varieties of B.benariensis‘BIG’grown in China rely on foreign imports.MS and 1/2MS were used as basic media,and combined with different plant hormones respectively to study the effects of combination media on adventitious bud differentiation,regeneration and rooting of B.benariensis,so as to establish a rapid propagation system for B.benariensis,and to solve the germplasm resource dilemma in production.The results showed that the optimal medium for adventitious bud differentiation was MS+1 mg/L 6-BA+0.1 mg/L 2.4-D+0.2 mg/L NAA.The optimal medium for adventitious bud subculture was MS+1 mg/L 6-BA+0.1 mg/L NAA.The optimal medium for rooting was 1/2MS+1.0 mg/L NAA.The experiment established a tissue culture regeneration system for B.benariensis‘BIG’,which provided a theoretical basis for large scale culture and propagation.