以污泥水解液作为生物法处理偶氮染料废水的共代谢基质研究

Treating Azo Dye Wastewater With Sludge Hydrolysate as Co-metabolic Substrate

本研究针对偶氮染料RB5在生物处理过程中的难降解问题,探究了以剩余污泥水解液作为混合菌群共代谢基质降解RB5的可行性。研究首先在T=30℃、pH=13及t=8h、10h、12h、14h、16h条件下制备出污泥水解液,分析水解液的基本成分,随后将其用作混合菌群的共代谢基质处理RB5。结果表明:COD溶出率(DDCOD)随水解时间的延长而升高,水解16h,DDCOD达到51.9%,蛋白质和多糖含量呈现波动趋势;混合菌群以处理12h的水解液作为共代谢基质时,RB5脱色效果最好,反应达到平衡时脱色率、COD去除率和OD600分别为90.5%、42.9%和0.887;FTIR和GC-MS结果表明水解液中含有较多酸类、醇类、酯类和烷烃类等物质。研究证明污泥水解液可以有效地作为混合菌群降解RB5的共代谢基质,为此类偶氮染料废水的处理及污泥资源化利用提供新思路。

This study explores the feasibility of using hydrolysate of waste activated sludge as a co-metabolic substrate to treat wastewater con-taining refractory RB5 azo dyes.Sludge hydrolysate was prepared at T=30℃,pH=13 after hydrolyzing for 8,h 10 h,12 h,14 h,and 16 h,and their components were analyzed.Afterwards,the sludge hydrolysate was used as co-metabolic substrates for decolorization of RB5 dye.The results showed that the COD dissolution efficiency(DDCOD)increased with the extension of hydrolysis time,with a DDCOD of 51.9%after hydrolyzing for 16 h,while the contents of proteins and polysaccharides fluctuated during sludge hydrolysis.The acclimated microbial consortium exhibited the highest RB5 de-colorization performance with the sludge hydrolysate obtained after hydrolyzing for 12 h as a co-metabolic substrate.When reaching reaction equilib-rium,the decolorization efficiency,COD removal efficiency,and OD600 were 90.5%,42.9%,and 0.887,respectively.The FTIR and GC-MS results in-dicated that the sludge hydrolysate contained a large number of acids,alcohols,esters,and alkanes.This research shows that sludge hydrolysate can effectively serve as a co-metabolic substrate for RB5 decolorization,and provides new ideas for the treatment of azo dyes and the reclamation of waste activated sludge.