WRAP53与甲状腺乳头状癌患者临床病理特征及预后的关系

The relationship between WRAP53 and clinicopathological features and prognosis of thyroid papillary carcinoma patients

目的:探讨端粒酶新核心亚单位(WD40-encoding RNA antisense to p53,WRAP53)又名端粒酶卡哈尔体蛋白1(telomerase cajal body protein 1,TCAB1)在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)组织中的表达,分析其与患者临床病理特征及预后的关系。方法:收集28例PTC患者的癌及癌周正常新鲜组织标本,应用实时荧光定量聚合酶链反应法(quantitative real-time polymerase chain reaction,qPR-PCR)及免疫组织化学法分别检测WRAP53 mRNA及蛋白在PTC及周围正常组织中的表达,收集117例PTC术后患者的临床数据及对应的癌组织的石蜡切片进行免疫组化检测,分析其蛋白表达水平与甲状腺乳头状癌患者的临床病理特征的相关性,最后应用蛋白质表达图谱(The Human Protein Atlas,HPA)数据库探究WRAP53与PTC预后之间的关系。结果:qPR-PCR及免疫组化结果提示WRAP53在PTC中较正常组织高表达(P<0.05)。与WRAP53低表达的PTC患者相比,WRAP53高表达的PTC患者肿瘤直径更长,更易出现淋巴结转移(P<0.05)。HPA数据库显示WRAP53高表达的PTC患者的生存率差于WRAP53低表达的PTC患者(P=0.041)。结论:通过本次研究表明,WRAP53在PTC组织中表达较周围正常组织升高,且其高表达与PTC增殖、淋巴结转移及不良预后相关。WRAP53可能是治疗PTC新的潜在靶点。

Objective:To investigate the expression of WD40-encoding RNA antisense to p53(WRAP53)also known as telomerase cajal body protein 1(TCAB1) in papillary thyroid carcinoma(PTC) and its relationship with clinical pathological characteristics and prognosis.Methods:Fresh tissue samples of PTC and adjacent normal tissues were collected from 28 patients,and quantitative real-time polymerase chain reaction(qPR-PCR) and immunohistochemistry were used to detect the expression of WRAP53 mRNA and protein in PTC and surrounding normal tissues.Clinical data and corresponding paraffin sections of cancer tissues were collected from 117 PTC patients after surgery for immunohistochemistry detection,and the correlation between the protein expression level and clinical pathological characteristics of PTC was analyzed.Finally,The Human Protein Atlas(HPA) database was used to explore the relationship between WRAP53 and the prognosis of PTC.Results:The results of qPR-PCR and immunohistochemistry showed that WRAP53 was overexpressed in papillary thyroid carcinoma(PTC) compared to normal tissues(P<0.05).Compared with PTC patients with low expression of WRAP53,PTC patients with high expression of WRAP53 had larger tumor size and were more likely to have lymph node metastasis(P<0.05).The HPA database showed that the survival rate of thyroid cancer patients with high expression of WRAP53 was lower than that of patients with low expression of WRAP53,and the difference was statistically significant(P=0.041).Conclusion:This study demonstrates that WRAP53 is overexpressed in PTC tissues compared to surrounding normal tissues,and its high expression is associated with proliferation,lymph node metastasis,and poor prognosis of PTC.WRAP53 may be a new potential therapeutic target for the treatment of PTC.