IL-13、IL-4、ADRβ2、FcεRI-β单核苷酸多态性与儿童反复喘息发作疗效及临床转归的关系

Correlation of IL-13,IL-4,ADRβ2,FcεRI-βsingle nucleotide polymorphism with curative effect and clinical outcome of relapse wheezing in children

目的了解白细胞介素-13(IL-13)、白细胞介素-4(IL-4)、肾上腺素能受体β2(ADRβ2)、免疫球蛋白E高亲和力受体β链(FcεRI-β)单核苷酸多态性(SNP)与儿童反复喘息发作的疗效及临床转归的关系。方法选取60例喘息性支气管炎反复喘息发作患儿作为研究对象,设计IL-13 rs20541、IL-4 rs2243250、ADRβ2 R16G、FcεRI-βE237G位点的引物,检测IL-13、IL-4、ADRβ2、FcεRI-β基因多态性,分析各基因位点SNP与患儿疗效及临床转归的关系。结果IL13 rs20541野生型AA和杂合型GA的治疗总有效率高于纯合型GG(P<0.01);ADRβ2 R16G不同基因型之间的治疗有效率比较差异有统计学意义(P<0.01),其中基因型GG的治疗有效率显著高于基因型AA、AG(P<0.05);FcεRI-βE237G治疗有效率比较AA>AG>GG(P<0.05)。IL-13 rs20541野生型AA和杂合型GA发生持续性哮喘的概率低于纯合型GG(P<0.05);ADRβ2 R16G不同基因型间的疾病转归比较差异有统计学意义(P<0.05),其中基因型GG发生持续性哮喘的概率显著低于基因型AA、AG(P<0.05);FcεRI-βE237G发生持续性哮喘的概率GG>AA>AG(P<0.05)。结论IL-13、ADRβ2、FcεRI-βSNP与反复喘息发作患儿的疗效及临床转归有关。

Objective To explore the correlation between IL-13,IL-4,ADRβ2,and FcεRI-βsingle nucleotide polymorphism(SNP)and the curative effect and clinical outcome of relapse wheezing in children.Methods A total of 60 children with relapse wheezing of asthmatic bronchitis were selected as the research subjects.The primes of IL-13 rs20541,IL-4 rs2243250,ADRβ2 R16G and FcεRI-βE237G sites were designed to test the SNP of IL-13,IL-4,ADRβ2 and FcεRI-β,and the correlation of SNP of each genetic locus with the curative effect and clinical outcome in children was analyzed.Results The total effective rates of IL-13 rs20541 wild type AA and heterozygous type GA was higher than that of homozygous type GG(P<0.01).There were statistical differences in the effective rate among different genotypes of ADRβ2 R16G(P<0.01),of which the effective rate of the genotype GG was higher than that of the genotype AA and AG(P<0.05).for the comparison of the effective rate of different genotypes of FcεRI-βE237G,AA>AG>GG(P<0.05).The incidence of persistent wheezing of IL-13 rs20541 wild type AA and heterozygous type GA was lower than that of homozygous type GG(P<0.05).There were statistical differences in the outcome among different genotypes of ADRβ2 R16G(P<0.05),of which the incidence of persistent wheezing of the genotype GG was higher than that of the genotype AA and AG(P<0.05).For the incidence of persistent wheezing of different genotypes of FcεRI-βE237G,GG>AA>AG(P<0.05).Conclusion IL-13 rs20541,ADRβ2 R16G,and FcεRI-βE237G SNPs are correlated with the curative effect and clinical outcome of children.