碳源及基因型对粳稻花药培养效果的影响

Effects of Different Carbon Sources and Genotypes on Anther Culture Efficiency of Japonica Rice

为了优化粳稻花药培养技术体系,提高粳稻杂交组合的花药培养效率,选用2种碳源麦芽糖、蔗糖设置不同处理的诱导培养基对10个粳稻F 4杂交组合进行花药培养。结果表明,粳稻花药培养过程中诱导培养基添加蔗糖花药培养效果要优于麦芽糖。然后用优化的诱导愈伤培养基对6个粳稻不同世代杂交组合进行花药培养,其中组合J118表现出较好的愈伤组织诱导率(9.90%)和绿苗分化率(14.48%),花药培养力最高(1.43%)。因此,J118是花药培养再生能力强的杂交组合,可作为单倍体育种、基因定位与克隆和遗传转化研究的基础材料,对提高花培育种效率和加速新品种育成具有重要意义。

In order to optimize the Japonica rice anther culture system and improve the anther culture efficiency of Japonica rice hybrid combinations,two carbon sources maltose and sucrose were selected in this study to set up induction medium of different treatments for anther culture of 10 Japonica rice F 4 hybrid combinations.The results showed that the induction medium with sucrose was more effective than maltose in Japonica rice anther culture.Then six Japonica rice hybrid combinations of different generations were cultured with optimized callus induction medium,in which combination J118 showed better callus induction rate(9.90%)and green seedling differentiation rate(14.48%),and the highest anther culture ability(1.43%).Therefore,J118 was a hybrid combination with strong regeneration ability in anther culture,which could be used as the basic material suitable for haploid breeding,gene mapping and cloning,genetic transformation research.It was of great significance to improve the efficiency of anther culture breeding and accelerate the breeding of new varieties.