新型大鼠腰部慢性骨骼肌复合损伤模型的建立

Establishment of a novel composite rat model of chronic skeletal muscle injury

目的本研究旨在建立一种新型大鼠腰部慢性骨骼肌复合损伤模型,以深入探索其机制和治疗方法。方法将28只SD大鼠随机分为对照组(10只)、单纯打击伤模型组(打击组,垂直打击)9只、复合损伤模型组(复合组,垂直打击结合强迫长时坐位)9只,造模3 d后行旷场实验、HE染色和PCR测序。结果打击组大鼠旷场路程明显低于复合组,两组均低于对照组。HE染色提示打击组多处肌纤维断裂,肌间隙明显增宽,肌核分布不均,可见充血;复合组肌纤维断裂,肌间隙稍增宽,肌间可见炎症细胞浸润,特别是肌纤维断裂周围有肌纤维核聚集。测序结果中火山图提示Pf4基因具有显著差异性。复合组大鼠肌肉组织GO分析中氧气结合和氧载体活性通路上调。结论本研究尝试建立了一种复合骨骼肌损伤模型,该模型可用于研究腰部慢性骨骼肌损伤的病理机制,并可用于评估治疗方法的疗效,在慢性非特异性腰痛的动物实验中具有实际应用价值。

Objective To establish a novel rat model of chronic skeletal muscle composite injury in the lumbar region,to explore its mechanism and treatment method in depth.Methods Twenty-eight SD rats were randomly divided into control group(n = 10),Hitting injury model(HIM) group(n = 9),Composite damage model(CDM) group(n = 9)(vertical blow combined with forced prolonged sitting).Open field test,HE staining and PCR sequencing were performed 3 days after modeling.Results The open field distance of rats in HIM group was significantly shorter than that in the CDM group,and both groups had shorter distances than the control group.HE staining showed multiple muscle fiber breaks,significant widening of muscle gaps,uneven distribution of muscle nuclei,and visible congestion in the model group of simple blow injury;In the CDM group,muscle fibers were ruptured,the muscle gaps were slightly widened,and inflammatory cell infiltration was observed between the muscles,especially around the muscle fiber breakage,with muscle fiber nuclei gathering.A volcano plot of the sequencing result suggested significant differences in the Pf4 gene.GO analysis of muscle tissue in model group rats showed upregulation of oxygen binding and carrier activity pathways.Conclusions This study attempted to establish a composite skeletal muscle injury model to study the pathological changes of chronic skeletal muscle injury in the low back pain and evaluate the efficacy of treatment method.It has practical application value in animal experiments for chronic non-specific low back pain.