葛根多糖对猫肠道菌群的影响

Effects of Pueraria lobata polysaccharides on intestinal microflora of cats

为探究葛根多糖(PPL-1)对猫肠道菌群的影响,本试验选取8只体重为2.5~3.7 kg的家猫,按体重随机分为2组,包括对照组(W)和试验组(E),每组4只(两只公猫,两只母猫),试验组猫连续口服30 d葛根多糖后,收集粪便,通过IlluminaMiseq高通量测序技术对肠道菌群的多样性进行分析。结果显示:葛根多糖能够显著提高变形杆菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、罕见小球菌属(Subdoligranulum)、双歧杆菌属(Bifidobacterium)的相对丰度;显著降低厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)的相对丰度。结果表明,葛根多糖能显著提高猫肠道菌群多样性,并改善肠道菌群结构,对机体产生有益影响。

In order to explore the effects of Pueraria lobata polysaccharides(PPL-1)on intestinal flora of domestic cats,8 domestic cats with the body weight of 2.5~3.7 kg were selected and randomly divided into 2 groups according to body weight,with 4 cats in each group(two male cats and two female cats),including control group(W)and experimental group(E).Feces of the experimental group were collected after continuous oral administration of PPL-1 for 30 days.The diversity of intestinal flora was analyzed by Illumina Miseq high-throughput sequencing technology.The results showed that the polysaccharide could significantly improve the relative abundance of Proteobacteria,Bacteroidetes,Subdoligranulum and Bifidobacterium.The relative abundance of Firmicutes and Actinobacteria was significantly reduced.The results indicated that PPL-1 could significantly improve the diversity and structure of intestinal flora in cats,and had beneficial effects on the body.