摘要
通过改进紫外分光光度法对羊杂碎中亚硝酸盐进行测定,羊杂碎试样经醋酸-水(8∶2,V/V)溶液沉淀蛋白,75℃水浴超声提取25 min后过滤。通过显色剂对氨基苯磺酸-酸萘乙二胺偶合呈紫红色,在538 nm波长处检测吸光度测定亚硝酸含量。结果表明,亚硝酸盐在0.02~0.20μg·mL^(-1)线性关系良好,相关系数为0.99825,试样在3个不同含量水平(0.20 mg·kg^(-1)、0.40 mg·kg^(-1)、1.00 mg·kg^(-1))下的加标回收率为85.8%~90.3%,RSD为0.1%~3.9%,方法检出限为0.30 mg·kg^(-1),定量限为0.80 mg·kg^(-1)。该方法简便、提取效率高、准确性好、灵敏度高,适用于羊杂碎及羊杂汤中低亚硝酸盐含量的检测。
Nitrite in haggis was determined by an improved ultraviolet spectrophotometric method,and the haggis sample was filtered after precipitation of proteins by acetic acid-water(8∶2,V/V)solution and ultrasonic extraction for 25 min at 75℃in a water bath.The content of nitrite was determined by detecting the absorbance at 538 nm through the coupling of the chromogenic agent p-aminobenzene sulfonic acid-acid naphthalene ethylenediamine to a purplishred color.The results showed that the linearity of nitrite was good in the concentration range of 0.02~0.20μg·mL^(-1)with the correlation coefficient of 0.99825,and the recoveries of the specimens spiked at three different levels(0.20 mg·kg^(-1),0.40 mg·kg^(-1),1.00 mg·kg^(-1))ranged from 85.8%to 90.3%with the RSD of 0.1%~3.9%.The limit of detection was 0.30 mg·kg^(-1)and the limit of quantification was 0.80 mg·kg^(-1).The method is simple,with high extraction efficiency,good accuracy and high sensitivity,and is suitable for the determination of nitrite in haggis and haggis soup.
作者
孙小龙
樊惠华
米林锋
毛敏
侯磊磊
李月月
方莹
SUN Xiaolong;FAN Huihua;MI Linfeng;MAO Min;HOU Leilei;LI Yueyue;FANG Ying(Yulin Food Inspection and Testing Center,Yulin 719000,China)
出处
《现代食品》
2023年第21期169-172,共4页
Modern Food