家蚕雌蛹及其卵巢脂肪酸丰度分析

An Analysis of the Abundance of Fatty Acids in Silkworm Female Pupa and Ovary

研究使用气相色谱分析了家蚕雌蛹蛹体和卵巢中的脂肪酸丰度.结果显示,蛹化后的第1 d至第6 d,蛹体脂肪酸丰度的变化幅度小,第6 d至第9 d,蛹体脂肪酸丰度的变化幅度大.蛹化期间,主要脂肪酸丰度总体呈下降趋势,微量脂肪酸丰度呈上升趋势.卵巢脂肪酸丰度的变化幅度随发育递减.不同主要脂肪酸的变化幅度和方向各异,其中不饱和脂肪酸丰度增加,饱和脂肪酸丰度下降.微量脂肪酸丰度随发育明显下降,这表明微量脂肪酸与雌性生殖关联较小.蛹体和卵巢脂肪酸丰度的差异随发育逐渐缩小,至蛹化第6 d,卵巢中绝大部分的脂肪酸丰度与刚蛹化时有很大差异,但整体上与蛹体丰度相似,说明卵巢脂肪酸的积累依赖蛹体脂肪酸.蛹化后期,卵巢中丰度最高的α-亚麻酸出现下降,丰度次高的油酸明显增加,这可能与卵壳生成和卵子成熟有关;亚油酸在蛹化第6 d丰度差异最大,其在卵巢中的丰度显著增加并明显高过蛹体,表明卵巢具有为其发育以及卵子发生积累脂肪酸的特性.研究结果可为深入研究脂肪酸在家蚕和其他昆虫雌性生殖中的作用机制提供重要线索.

The study utilized gas chromatography analyzing the abundance of fatty acids in the bodies and ovaries of female silkworm pupae.The results revealed that the variation range of fatty acid abundance in the pupal body was small at first six days of pupation but increased from the sixth to ninth day.Overall,the main fatty acids exhibited a decreasing trend,while the trace fatty acids showed an increasing trend.The variation range of fatty acid abundance in the ovaries decreased with development.Different main fatty acids exhibited diverse patterns and directions of change,with an increase in unsaturated fatty acid abundance and a decrease in saturated fatty acid abundance.The abundance of trace fatty acids significantly decreased with development,indicating a less association between trace fatty acids and female reproduction.The differences in fatty acid abundance between the pupal body and ovaries gradually diminished with development.By the sixth day of pupation,most of fatty acids in the ovaries differed significantly from those observed during the initial pupation stage,but were similar to those in the pupal body,suggesting the dependence of fatty acid accumulation in ovary on the pupal body fatty acids,which is likely related to ovarian development and vitellogenesis.In the later stages of pupation,the highest abundance ofα-Lindenic acid fatty acid exhibited a decrease,while the second highest abundance of Oleicacid fatty acid significantly increased,which may be associated with eggshell formation and oocyte maturation.Linoleic acid displayed the greatest difference on the sixth day of pupation,with a significantly higher abundance in the ovaries than in the pupal body,indicating the specificity of accumulation of fatty acids in ovary during development and oogenesis.These results provide important clues for further investigating the mechanisms underlying the role of fatty acids in female reproduction of silkworms and other insects.