摘要
目的:建立一种猪支原体肺炎RT-PCR诊断方法。方法:选择云南省大理地区患有猪支原体肺炎疾病的60只猪作为研究对象,针对猪肺炎支原体的保守序列p36基因设计了引物和探针,建立猪支原体肺炎RT-PCR检测反应体系,并对其灵敏度、特异性、重复性进行分析及临床样本验证。结果:RT-PCR检测灵敏度为10^(2)拷贝、与PRRSV、PPV、FMDV、PCV、CSFV、APP等无交叉反应,批内和批间变异系数均小于2.00%,肺组织样本检出阳性数26例(86.67%),鼻拭子样本检出阳性数19例(63.33%)。结论:该研究成功建立了一种猪支原体肺炎RT-PCR诊断方法,具有较高的检测灵敏度和特异性,重复性能良好,这为后期MPS的临床精准诊断和疫病科学防控提供试验依据。
Objective:To establish a RT-PCR diagnostic method for Mycoplasma pneumonia of swine.Method:Sixty pigs suffering from Mycoplasma pneumonia of swine disease in Dali,Yunnan Province were selected as the research subjects.Primers and probes were designed for the conserved sequence p36 gene of Mhp,and a RT-PCR detection reaction system for Mycoplasma pneumonia of swine was established.The sensitivity,specificity,and repeatability were analyzed and clinical sample validation was conducted.Result:The sensitivity of RT-PCR detection was 10^(2) copies,and there was no cross reaction with PRRSV,PPV,FMDV,PCV,CSFV,APP,etc.The coefficient of variation within and between batches was less than 2.00%.Positive cases were detected in 26 lung tissue samples(86.67%)and 19 nasal swab samples(63.33%).Conclusion:This study has successfully established a RT-PCR diagnostic method for Mycoplasma pneumonia of swine,which has high detection sensitivity and specificity,and good repeatability.This provides experimental basis for the accurate clinical diagnosis and scientific disease prevention and control of MPS in the later stage.
作者
汪志恒
王景成
佘志成
王凯
WANG Zhiheng;WANG Jingcheng;SHE Zhicheng;WANG Kai(Hainan Luoniushan Animal Husbandry Co.,Ltd,Haikou Hainan 570125,China;Jinyu Baoling Biopharmaceutical Co.,Ltd,Hohhot Inner Mongolia 010030,China)
出处
《现代畜牧科技》
2024年第1期6-9,共4页
Modern Animal Husbandry Science & Technology
基金
国家生猪产业体系海口综合试验站(CARS-35)。
