安钠咖对人脐静脉血管内皮细胞损伤的研究

Study on Caffeine Sodium Benzoate-induced Injury of Human Umbilical Vein Endothelial Cells

目的安钠咖属于精神类药品,长期大剂量使用对使用者的身心健康影响极大,除导致成瘾外,还易诱发多种疾病。该研究探讨安钠咖介导内皮细胞功能异常进而导致凝血功能障碍的机制。方法基于临床数据探讨安钠咖对凝血功能及相关疾病的影响、β-半乳糖苷酶染色观察用25%安钠咖患者血清+75%正常人血清、50%安钠咖患者血清+50%正常人血清、75%安钠咖患者血清+25%正常人血清、安钠咖患者血清以及脂多糖刺激人脐静脉血管内皮细胞(HUVECs)后的情况并拍照对比,以及通过HUVECs成管实验观察安钠咖血清对HUVECs形成血管能力的影响。结果通过对临床数据的分析发现长期吸食安钠咖会严重影响患者的凝血功能,并且随着安钠咖血清占比增加出现阳性细胞占比增多(P<0.05)即衰老细胞增多,以及通过细胞成管实验发现安钠咖患者血清刺激后的HUVECs血管形成率下降(P<0.05)。结论长期吸食安钠咖会导致患者血管内皮细胞功能异常进而引发凝血功能障碍。

Objective Long-term use of large-dose caffeine sodium benzoate,a psychotropic drug,has a great impact on the physical and mental health of users,leading to not only addiction but also varieties of diseases.This study was to investigate the mechanism of endothelial cell dysfunction mediated by caffe natrium causing coagulation dysfunction.Methods Based on clinical data,the effects of sodium ampoule on coagulation function and related diseases were examined.β-galactosidase staining was done to observe the stimulation of human umbilical vein endothelial cells(HUVECs)using 25%sodium cafe patient serum+75%normal human serum,50%sodium cafe patient serum+50%normal human serum,75%sodium cafe patient serum+25%normal human serum,sodium cafe patient serum and lipopolysaccharide,and the effects of sodium ampoule serum on HUVECs were analyzed via the HUVEC cell tube experiment to test the ability of cells to form blood vessels.Results Long-term consumption of caffeine sodium benzoate seriously affected the coagulation function of patients,and the proportion of positive cells increased with higher coffee with sodium ampna serum(P<0.05),i.e.senescent cells were elevated,and cell tube experiments showed that HUVECs blood vessel formation rate decreased after serum stimulation in patients with caffeine sodium benzoate(P<0.05).Conclusion Long-term consumption of caffeine sodium benzoate can lead to abnormal function of vascular endothelial cells in patients and hence coagulation dysfunction.