冰点冷却倒置离心法降低冰冻血浆乳糜程度的研究

The effect of freezing point cooling Inverted centrifugation method on frozen plasma chylemia reduction

目的分析冰点冷却倒置离心法降低中、重度乳糜血浆乳糜程度的效果。方法收集本站报废的冰冻血浆46份,采用冰点冷却倒置离心法去除血浆中的乳糜微粒,分析处理前后2组血浆的吸光度、甘油三脂(TG)、胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、乳糜指数和血浆总蛋白(TP)并评价其处理效果。结果中度乳糜血浆处理后其OD值、TG和乳糜指数均明显降低(P<0.01),而胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)和TP含量与处理前无统计学差异(P>0.05)。重度乳糜血浆经冰点冷却倒置离心处理后其OD值、TG和乳糜指数均明显降低(P<0.01),外观明显改善,综合达标符合率为75%;而胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)和TP含量与处理前无统计学差异(P>0.05)。结论冰点冷却倒置离心法可以有效降低血浆乳糜程度,提高血浆质控达标符合率,尽可能减少血液资源的浪费。

Objective The aim of this study is to explore the efficiency of freezing point cooling inverted centrifugation on plasma chylemia reduction.Methods 46 bags of frozen plasma(200 mL/bag)were collected from blood composition preparation process.The freezing point cooling inverted centrifugation was employed for removal of chylemia from plasma,and the absorbance,lipid levers,total plasma protein(TP)and chylemia were analyzed before or after the centrifugation to evaluate the effect of chylemia reduction.Results After the treatment of freezing point cooling inverted centrifugation,the OD value,TG content and chylous index of were significantly decreased in moderate chylous plasma(P<0.01).While,the levels of TC,HDL,LDL and TP were not significantly different from those without treatment(P>0.05).The OD value,TG content and chylous index were significantly decreased in severe chylous plasma after treatment of freezing point cooling inverted centrifugation(P<0.01).The appearance of plasma was significant improved,and the compliance rate of comprehensive standards was 75%.However,the levels of TC,HDL,LDL and TP were not significantly different from those without treatment(P>0.05).Conclusion Freezing point cooling inverted centrifugation method can effectively reduce plasma chyle degree,improve the compliance rate of plasma quality control,reduce the scrap rate of blood and minimize the waste of blood resources as much as possible.