真菌诱导子调控紫草素合成的分子机制探究

Explore of Molecular Mechanism on Fungal Elicitors Regulating Shikonin Synthesis

为探讨真菌诱导子调控紫草素合成的分子机制,以新疆紫草无菌苗为试材,经尖孢镰刀菌、立枯丝核菌制备成的诱导子生物诱导后,对其根部进行RNA测序和分析。结果表明,与对照组比较,尖孢镰刀菌试验组差异表达基因1735个;立枯丝核菌试验组差异表达基因1043个。GO(gene ontology)分析发现,2个试验组差异表达基因主要富集在细胞过程、细胞组分中膜和分子功能中催化活性等生物学过程。KEGG(kyoto encyclopedia of genes and genomes)分析发现,2个试验组在植物与病原菌互作、植物激素信号转导途径和苯丙素合成等通路均有大量差异表达基因富集。2个试验组差异表达情况相同的转录因子主要包括bHLH、AP2/ERF-ERF和LOB等,并发现参与紫草素合成及其正向调控的AeGHQH、AeDSH1、AeAP、AePAL、AeDI2、AePGT、AeHMGR、AeG10H基因在2个试验组均上调表达,其中尖孢镰刀菌试验组上调表达较显著。以上结果从分子水平探究了新疆紫草对真菌诱导子生物诱导的响应机制,为未来真菌诱导子应用于新疆紫草种植生产奠定了理论基础。

To explore the molecular mechanism of fungal elicitors regulating shikonin synthesis,RNA sequencing and analysis were performed on the roots of Arnebia euchroma sterile seedlings after bio-induction by elicitors prepared from Fusarium oxysporum and Rhizoctonia solani.The results showed that,compared with the control group,the Fusarium oxysporum experimental group differentially expressed 1735 genes;the Rhizoctonia solani experimental group differentially expressed 1043 genes.GO(gene ontology)analysis revealed that the differentially expressed genes of 2 experimental groups were mainly enriched in cellular processes in biological processes,membrane in cellular components,and catalytic activity in molecular functions.KEGG(kyoto encyclopedia of genes and genomes)analysis revealed that 2 experimental groups were enriched in a large number of differentially expressed genes in the pathways of plant-pathogen interaction,phytohormone signaling pathways and phenylpropanoid synthesis.The same transcription factors were differentially expressed in 2 experimental groups,mainly including bHLH,AP2/ERF-ERF and LOB.The expression of AeGHQH,AeDSH1,AeAP,AePAL,AeDI2,AePGT,AeHMGR and AeG10H genes,which were involved in the synthesis and positive regulation of shikonin,were found to be up-regulated in 2 experimental groups,with more significant up-regulation in the Fusarium oxysporum experimental group.Above results elaborated the response mechanism of Arnebia euchroma in the biological induction of fungal elicitors at the molecular level,which laid a theoretical foundation for the future application of fungal elicitors in the cultivation and production of Arnebia euchroma.