基于网络药理学探讨附子-半夏药对治疗缺血性中风的作用机制

Study on Mechanism of Fuzi(Aconiti Lateralis Radix Praeparata)-Banxia(Pinelliae Rhizoma)in Treating Ischemic Stroke Based on Network Pharmacology

目的运用网络药理学方法探讨附子-半夏药对治疗缺血性中风的作用机制。方法通过TCMSP数据库收集药对的有效成分及作用靶点信息;运用Uniprot蛋白质数据库寻找蛋白质靶点和相应的人类基因;利用GeneCards数据库和OMIM数据库预测疾病靶点,运用Venny2.1取二者交集靶点;利用STRING、Cytoscape3.7.2绘制PPI网络;最后通过R软件进行GO以及KEGG分析,并使用Cytoscape3.7.2软件构建“成分-疾病靶点-通路图”。结果附子-半夏药对共同拥有β-谷甾醇、黄芩苷、豆甾醇、卡文定碱、松柏苷等主要活性成分通过脂质、粥样硬化、细胞凋亡及P53信号通路作用在AKT1、CASP3、PTGS2、TP53、PPARG等靶点上治疗缺血性中风。结论附子-半夏药对通过多靶点、多途径、多通路发挥防治缺血性中风的作用。

Objective To study the mechanism of Fuzi(Aconiti Lateralis Radix Praeparata)-Banxia(Pinelliae Rhizoma)in the treatment of ischemic stroke by network pharmacology.Method It collected the information of active components and action targets of drug pairs through TCMSP database and used the Uniprot protein database to find out protein targets and corresponding human genes.GeneCards database and OMIM database were used to predict disease targets,and Venny 2.1 was used to get the intersection targets of the two.Drawing PPI network was carried out with STRING and Cytoscape 3.7.2 Finally,GO and KEGG were analyzed by R software and"component disease-target-pathway map"was constructed by using Cytoscape 3.7.2 software.Result Fuzi(Aconiti Lateralis Radix Praeparata)-Banxia(Pinelliae Rhizoma)passed the main active components such as beta-sitosterol,baicalin,stigmasterol,cavidine and coniferin act on AKT1,CASP3,PTGS2,TP53,PPARG and other targets to treat ischemic stroke through lipid and atherosclerotic,apoptosis and P53 signaling pathways.Conclusion Fuzi(Aconiti Lateralis Radix Praeparata)-Banxia(Pinelliae Rhizoma)has the characteristics of multiple targets,multiple pathways and multiple pathways in the treatment of ischemic stroke.