八宝五胆药墨抑制皮肤感染炎症反应作用研究

Study on effect of Babao Wudan Ink Pastille in inhibiting inflammatory reaction of skin infection

目的:观察八宝五胆药墨对小鼠皮肤感染炎症反应的作用。方法:将雌性Balb/c小鼠随机分为正常对照组、模型组、低剂量八宝五胆药墨组、高剂量八宝五胆药墨组和阳性药对照组,造模成功后分别给予相应的干预措施,4周后通过苏木精-伊红(HE)染色观察各组小鼠皮肤病变组织的形态结构,采用酶联免疫吸附剂测定(ELISA)法测定血清白介素(IL)-1、IL-6、肿瘤坏死因子-α(TNF-α)的含量。结果:低剂量八宝五胆药墨组和阳性药对照组小鼠血清IL-1、IL-6、TNF-α水平较模型组均降低(P<0.05),高剂量八宝五胆药墨组小鼠血清IL-1、IL-6、TNF-α较模型组明显降低(P<0.01),且高剂量八宝五胆药墨组小鼠血清IL-1、IL-6、TNF-α水平较低剂量八宝五胆药墨组和阳性药对照组均降低(P<0.05)。结论:八宝五胆药墨可能通过抑制炎症因子IL-1、IL-6、TNF-α的释放减轻小鼠皮肤感染损伤的程度。

Objective:To observe the effect of Babao Wudan ink pastille on inflammatory reaction of skin infection in mice.Methods:Female Balb/c mice were randomly divided into normal control group,model group,low-dose Babao Wudan ink pastille group,high-dose Babao Wudan ink pastille group,and positive drug control group.After successful modeling,corresponding intervention measures were given.After 4 weeks,the morphological structure of the skin lesion tissue of mice in each group was observed by HE staining,and the contents of Serum IL-1,IL-6,and TNF-αwere measured by ELISA.Results:Compared with the model group,the contents of Serum IL-1,IL-6,TNF-αof mice in low-dose Babao Wudan ink pastille group and positive control group were reduced(P<0.05);and the contents of Serum IL-1,IL-6 and TNF-αof mice in high-dose Babao Wudan ink pastille group were significantly reduced(P<0.01).In addition,the contents of serum IL-1,IL-6 and TNF-αof mice in the high-dose Babao Wudan ink pastille group were reduced compared with those of the lowe-dose Babao Wudan ink pastille group and the positive control group(P<0.05).Conclusion:Babao wudan ink pastille can reduce the degree of skin infection damage in mice by inhibiting the release of the inflammatory factors of IL-1,IL-6,TNF-α.