长日照条件下九叶期玉米的蛋白质组学分析

Proteomic analysis of maize at nine⁃leaf stage under long sunshine condition

【目的】探明长日照条件下玉米九叶期差异蛋白的特性,为揭示玉米的分子机理提供生物学基础。【方法】以LP光敏感(NT32)和光钝感(NQR273)玉米自交系为试验材料,采用人工控制长日照条件(16 h光/8 h暗)的方法对试验材料进行处理,于九叶期采集幼嫩叶片,利用TMT技术对不同光敏类型自交系材料进行蛋白质组学分析,同时结合生物信息学分析,研究其在长日照条件下差异蛋白的表达特性。【结果】长日照条件下玉米九叶期叶片中共检测到差异蛋白191个,其中上调表达107个,下调表达84个;GO富集分析显示,差异蛋白主要参与核糖体的结构组成、半胱氨酸型肽酶活性及锰离子结合过程等生物学过程。蛋白水解和翻译过程是重要的分子功能类别。差异蛋白主要富集于次生代谢物的生物合成、谷胱甘肽代谢、卟啉和叶绿素代谢等通路。网络互作分析筛选到氨基酸合成、核糖体代谢过程是玉米在长日照条件下生长发育的关键途径。有79个差异蛋白具有功能注释,分别参与ROS体内平衡(19个)、贮藏物质保护(7个)、氨基酸代谢(8个)、其他功能基因(45个)。【结论】光敏感NT32玉米在长日照条件下ROS清除能力增强,贮藏物质保护能力及氨基酸代谢下降,其他功能基因以不同的方式发挥作用,即在长日照条件下,光敏感NT32玉米叶片以氨基酸合成、核糖体代谢过程为主的调控系统,维持了玉米的稳定生长。

【Objective】The characteristics of differential proteins of maize at nine⁃leaf stage under long sunshine condition were investigated,so as to provide biological basis for revealing the molecular mechanism of maize.【Method】LP light sensitive(NT32)and light insensitive(NQR273)maize inbred lines were used as experimental materials.The experimental materials were treated with artificial control of long sun⁃shine(16 hours light/8 hours dark).Young leaves of maized were collected at the nine⁃leaf stage,and proteomic analysis of different light⁃sensitive inbred lines was analyzed by TMT technique.At the same time,bioinformatics analysis was used to study the expression characteris⁃tics of differential proteins under long sunshine condition.【Result】A total of 191 differential proteins were detected from maize at nine⁃leaf stage under long sunshine condition,of which 107 were up⁃regulated and 84 were down⁃regulated.GO enrichment analysis showed that differ ential proteins were mainly involved in biological processes such as ribosome structure,cysteine⁃type peptidase activity and manganese ion binding process.Interaction network analysis showed that proteolysis and translation processes were important molecular functional clas⁃ses.Differential proteins were mainly enriched with secondary metabolite biosynthesis,glutathione metabolism,porphyrin and chlorophyll me⁃tabolism and other pathways.Amino acid synthesis and ribosome metabolism were identified as key pathways druing the growth period of maize under long sunshine condition.There were 79 differential proteins with functional annotation,which were involved in ROS homeostasis(19),storage protection(7),amino acid metabolism(8),and other functional genes(45).【Conclusion】The ROS clearance ability of light⁃sensitive NT32 corn was enhanced under long sunshine condition,while the storage protection ability and amino acid metabolism de⁃creased,and other functional genes played their roles in different ways,which meant that the regulatory system of amino acid synthesis and ribosome metabolism in the leaves of light⁃sensitive NT32 maize maintained the stable growth of corn under long sunshine condition.