花鲈半乳糖凝集素基因家族的鉴定及在不同环境因子胁迫下表达响应

Identification of the Galectin Genes in Spotted Sea Bass(Lateolabrax maculatus)and Their Expressions Under Different Environmental Stressing

为探究在花鲈(Lateolabrax maculatus)响应环境因子胁迫中半乳糖凝集素(Galectins)发挥的作用,本研究通过全基因组水平鉴定获得了花鲈的14个Galectin基因(LGALS1、LGALS2a、LGALS2b、LGALS3a、LGALS3ba、LGALS3bb、LGALS4、LGALS8a、LGALS8b、LGALS9、LGALS17、GRPa、GRPb和GRPc)。通过系统发育、拷贝数、共线性和基因结构分析证实了基因注释的准确性和结构上的保守性。利用转录组测序数据进行Galectin基因的组织表达及在多种环境因子胁迫下的表达模式分析。研究表明,Galectin家族各基因在花鲈的7个组织(肝、鳃、脾、胃、脑、精巢和卵巢)中广泛表达,且有6个基因(LGALS3ba、LGALS4、LGALS8a、LGALS9、LGALS17和GRPa)表现出明显的组织特异性。Galectin家族的不同成员在4种环境因子胁迫下(低氧、碱度、高温及盐度适应)表现不同程度的表达响应,其中LGALS3a、LGALS3ba、LGALS4、LGALS17和GRPb在4种环境胁迫中表现出显著的差异表达,表明其广泛参与了对环境胁迫的响应与调控。

To investigate the roles of Galectin genes of spotted sea bass(Lateolabrax maculatus)in responding different environmental stressing,14 Galectin genes(LGALS1,LGALS2a,LGALS2b,LGALS3a,LGALS3ba,LGALS3bb,LGALS4,LGALS8a,LGALS8b,LGALS9,LGALS17,GRPa,GRPb and GRPc)were identified in this study from its genome.The accuracy of gene annotation and structural conservation were verified by phylogenetic,copy number,collinearity and gene structure analyses.RNA-seq data were used to analyze their tissue-specific expressions and their expressional response to different environmental stressing.Tissue-specific expression profiles indicated that the Galectin genes were widely expressed in 7 tissues(liver,gill,spleen,stomach,brain,testis and ovary),and 6 genes(LGALS3ba,LGALS4,LGALS8a,LGALS9,LGALS17 and GRPa)showed obvious tissue specificity.Different members of the Galectin gene family showed different levels of expression in responding four environmental factor stresses(hypoxia,alkalinity,heat and salinity acclimation),among them LGALS3a,LGALS3ba,LGALS4,LGALS17 and GRPb showed significantly differential expression in the four environmental stresses,indicating that their extensive involvement in the response and regulation of environmental stresses.