Fas/FasL传导通路研究电针对慢性萎缩性胃炎小鼠的作用机制

Fas/FasL Conduction Pathway Studies the Mechanism of Electroacupuncture on Chronic Atrophic Gastritis in Mice

目的探讨电针合募配穴对慢性萎缩性胃炎小鼠Fas/FasL传导通路的影响,揭示电针治疗慢性萎缩性胃炎的作用机制。方法制备慢性萎缩性胃炎小鼠模型。将已成模的慢性萎缩性胃炎小鼠分为模型组10只、普通针刺组、电针组各20只,并另设10只为对照组。对照组、模型组不进行操作;普通针刺组予合募配穴(中脘-足三里)普通针刺治疗;电针组予合募配穴(中脘-足三里)电针治疗,采用免疫组化法检测各组小鼠胃组织中Fas和FasL的表达水平。结果治疗前普通针刺组、电针组、模型组的体质量都显著低于对照组(P<0.05),治疗14 d后普通针刺组、电针组的体质量高于模型组(P<0.05),电针组治疗14 d后的体质量与对照组比较,差异无统计学意义(P>0.05)。治疗前普通针刺组、电针组、模型组的血清IL-6、IL-1β含量都高于对照组(P<0.05),治疗14 d后普通针刺组、电针组的血清IL-6、IL-1β含量低于模型组(P<0.05),电针组治疗14 d后的血清IL-6、IL-1β含量与对照组比较,差异无统计学意义(P>0.05)。治疗14 d后普通针刺组、电针组、对照组的胃黏膜组织病理形态评分与黏膜组织Fas蛋白、FasL蛋白相对表达水平都低于模型组(P<0.05),电针组与对照组比较,差异无统计学意义(P>0.05)。结论电针在慢性萎缩性胃炎小鼠的应用能介导Fas/FasL传导通路的表达,抑制血清IL-6、IL-1β的表达,促进恢复小鼠的体质量,能改善小鼠的胃黏膜病理状况。

Objective To explore the effects of electroacupuncture combined with acupoints Mu on Fas/FasL conduction pathway in mice with chronic Atrophic gastritis,and reveal the mechanism of electroacupuncture in treating chronic Atrophic gastritis.Methods The mouse model of chronic Atrophic gastritis were established.The modeled chronic Atrophic gastritis mice were divided into the model group of 10 cases,the common acupuncture group of 20 cases,and the electroacupuncture group of 20 cases,and another 10 cases of the control group.The control group and model group were not operated;The ordinary acupuncture group were treated with combined acupuncture at acupoints(Zhongwan Zusanli);The electroacupuncture group were treated with acupoint combination(Zhongwan Zusanli)electroacupuncture.Immunohistochemical methods were used to detect the levels of Fas and FasL in the gastric tissues of each groups of mice.Results The body weight of the ordinary acupuncture group,electroacupuncture group,and model group before treatment were significantly lower than that of the control group(P<0.05).After 14 days of treatment,the body weight of the ordinary acupuncture group and electroacupuncture group were higher than that of the model group(P<0.05).There were no significant difference in body weight between the electroacupuncture group and the control group after 14 days of treatment(P>0.05).Serum IL-6 and IL-1βin the ordinary acupuncture group,electroacupuncture group,and model group before treatment were higher than those in the control group(P<0.05).After 14 days of treatment,the serum IL-6 and IL-1βlevels in the ordinary acupuncture group and the electroacupuncture group were significantly higher than those in the control group,and were lower than that of the model group(P<0.05),and the serum IL-6 and IL-1βlevels in the ordinary acupuncture group after 14 days of treatment were lower than those in the model group(P<0.05),There were no significant difference in the electroacupuncture group of treatment compared to the control group(P>0.05).After 14 days of treatment,the histopathological score of Gastric mucosa and the relative expression level of Fas protein and FasL protein of mucosa in the ordinary acupuncture group,the electroacupuncture group and the control group were lower than those in the model group(P<0.05),and there were no significant difference compared between the electroacupuncture group and the control group(P>0.05).Conclusion The application of electroacupuncture in chronic Atrophic gastritis mice can mediate the expression of Fas/FasL conduction pathway,and can inhibit the serum IL-6,IL-1βThe expression of can also promote the recovery of mouse weight and improve the pathological condition of the gastric mucosa in mice.