綦江地区健康成人外周血T淋巴细胞亚群参考范围分析

Analysis of Reference Range of Peripheral Blood T Lymphocyte Subsets in Healthy Adults in Qijiang Area

目的探讨綦江地区健康成人外周血T淋巴细胞亚群的变化特点,进行统计分析,建立綦江地区T淋巴细胞亚群的实验室参考范围。方法选取2022年4月1日—2023年4月10日于綦江地区进行健康体检的311例体检者为研究对象,平均年龄为(32.35±5.83)岁。采集健康体检者清晨空腹静脉血,采用流式细胞术检测311例健康成人外周血T淋巴细胞亚群。结果男性总淋巴细胞绝对计数、总T淋巴细胞绝对计数、CD8^(+)淋巴细胞百分比、CD8^(+)淋巴细胞绝对计数高于女性,CD4^(+)淋巴细胞百分比低于女性,差异有统计学意义(P<0.05);两组总T淋巴细胞百分比、CD4^(+)淋巴细胞绝对计数、CD4^(+)/CD8^(+)比较,差异无统计学意义(P>0.05)。不同年龄组各项指标比较,差异无统计学意义(P>0.05)。采用百分位数法对311例健康体检者外周血T淋巴细胞亚群进行分析,并将各项指标的95%可信区间作为綦江地区健康成人外周血T淋巴细胞亚群的参考范围,具体如下:总淋巴细胞绝对计数1520×10^(6)/L~3780×10^(6)/L、总T淋巴细胞绝对计数950×10^(6)/L~2840×10^(6)/L、总T淋巴细胞百分比49%~85%、CD4^(+)淋巴细胞绝对计数545×10^(6)/L~1420×10^(6)/L、CD4^(+)淋巴细胞百分比26%~50%、CD8^(+)淋巴细胞绝对计数318×10^(6)/L~1255×10^(6)/L、CD8^(+)淋巴细胞百分比12%~42%、CD4^(+)/CD8^(+)为0.70~2.90。结论性别会影响健康成人外周血T淋巴细胞亚群的部分指标,建立适合本地区健康成人外周血T淋巴细胞亚群参考范围对疾病的诊断具有极其重要的意义。

Objective To investigate the changes of peripheral blood T lymphocyte subsets in healthy adults in Qijiang area,and to establish the laboratory reference range of T lymphocyte subsets in Qijiang area.Methods From April 1,2022 to April 10,2023,311 cases of physical examination in various hospitals in Qijiang area were selected as the subjects of this study.The average age was(32.35±5.83)years old.Venous blood was collected from healthy subjects with empty stomach in the morning,and T lymphocyte subsets in peripheral blood of 311 healthy adults were detected by fl ow cytometry.Results The absolute count of total lymphocytes,total T lymphocytes,percentage of CD8^(+)lymphocytes and absolute count of CD8^(+)lymphocytes in males was higher than that of females,and the percentage of CD4^(+)lymphocytes was lower than that of females,and the difference was statistically significant(P<0.05),while there was no significant difference in the percentage of total T lymphocytes,absolute count of CD4^(+)lymphocytes and CD4^(+)/CD8^(+)between the two groups(P>0.05).There was no significant difference in the indexes of different age groups(P>0.05).The peripheral blood T lymphocyte subsets of 311 healthy subjects were analyzed by percentile method,and the 95%confidence interval of each index was used as the reference range of healthy adults in Qijiang area.The results were as follows:total lymphocyte absolute count 1520×10^(6)/L~3780×10^(6)/L,total T lymphocyte absolute count 950×10^(6)/L~2840×10^(6)/L,total T lymphocyte percentage 49%~85%,CD4^(+)lymphocyte absolute count 545×10^(6)/L~1420×10^(6)/L,CD4^(+)lymphocyte percentage 26%~50%,CD8^(+)lymphocyte absolute count 318×10^(6)/L~1255×10^(6)/L,CD8^(+)lymphocyte percentage 12%~42%,CD4^(+)/CD8^(+)ratio 0.70~2.90.Conclusion Gender will affect some indicators of peripheral blood T lymphocyte subsets in healthy adults.It is of great significance to establish a reference range of peripheral blood T lymphocyte subsets suitable for healthy adults in the region for the diagnosis of diseases.