藜麦2,3-氧化角鲨烯环化酶基因(CqOSC)家族的全基因组鉴定与分析

Genome-Wide Identification and Analysis of 2,3-Oxidosqualene Cyclases Gene(CqOSC)Family in Quinoa(Chenopodium quinoa Willd.)

基于藜麦全基因组和转录组数据,利用生物信息学的方法对藜麦CqOSC基因家族进行了鉴定和系统分析。结果表明,在藜麦基因组中鉴定到15个CqOSC基因家族成员,可分为3个亚组,同亚组CqOSC基因具有相似的基因结构和蛋白Motif结构;在藜麦正常生长发育过程中,CqOSC基因家族成员的表达模式存在明显差异,CqOSC7和CqOSC12在花和未成熟的种子及干种子中显著表达,而CqOSC9与CqOSC10在所有组织中均呈现出一定水平的表达;对于MeJA具有不同响应:CqOSC2、CqOSC4和CqOSC8基因表达量变化显著;CqOSC3、CqOSC6和CqOSC15基因表达量变化不明显;整体表现为“低促高抑”:在外施0.5 mmol/L和1 mmol/L MeJA时基因表达量显著上升,在浓度达到2 mmol/L时表达量显著下降。

Based on quinoa genome-wide and transcriptome data,the CqOSC gene family of quinoa is identified and systematically analyzed by bioinformatics.The results show that 15 members of the CqOSCgene family are identified in the quinoa genome,which could be divided into three subgroups,and the same subgroup CqOSC genes have similar gene structure and protein motif structure.During the normal growth and development of quinoa,CqOSC7 and CqOSC12 are significantly expressed in flowers and immature seeds and dried seeds,while CqOSC9 and CqOSC10 show a certain level of expression in all tissues.There are different responses to MeJA:the expression of CqOSC2,CqOSC4 and CqOSC8 genes change significantly,in contrast to case of CqOSC3,CqOSC6 and CqOSC15.The overall performance is"low promotion and high inhibition":whereas gene expression increases significantly when 05 mmol/L and 1 mmol/L MeJA are applied externally,it decreases significantly when the concentration reaches 2 mmol/L.