BAP1在肝细胞肝癌中的表达及其与预后的关系

Expression of BAP1 in liver hepatocellular carcinoma and its relationship with prognosis

目的探讨BRCA1相关蛋白1(BAP1)在肝细胞肝癌(LIHC)中的表达变化及其与预后的关系。方法从UALCA获得癌症基因组图谱(TCGA)中LIHC的BAP1 m RNA表达水平及临床数据并进行数据分组和处理。采用R3.2.2软件进行分析。比较BAP1在癌组织与正常组织中的表达差异,并分析LIHC患者各亚组临床指标的BAP1表达水平与正常组织的差异。采用寿命表法计算生存率,采用Kaplan-Meier法比较BAP1高表达组和中低表达组患者的生存率,并绘制患者的生存曲线。结果LIHC组织中BAP1 mRNA表达中位数为37.748 TPM,明显高于正常组织中的18.444 TPM,差异有显著统计学意义(P<0.01);患者的性别、年龄、种族、体质量、组织学类型、组织学分级、淋巴结、TNM分期Ⅰ~Ⅲ期、TP53突变的各组癌组织中BAP1表达水平与正常组织表达水平比较差异均有统计学意义(P<0.05),而Ⅳ期组癌组织中BAP1表达水平与正常组织表达水平比较差异无统计学意义(P>0.05);患者的中位生存时间为27.18个月,1年、2年、3年、4年、5年生存率分别为0.57%、0.31%、0.20%、0.13%、0.08%;BAP1 m RNA高表达的整体生存率较BAP1mRNA中低表达者短,差异均有统计学意义(P<0.05)。结论BAP1 m RNA在LIHC中呈高表达,高表达组生存率较低,提示预后不良。

Objective To analyze the expression of BRCA1 associated protein 1(BAP1)in liver hepatocellular carcinoma(LIHC)and its relationship with prognosis.Methods BAP1 mRNA expression levels and clinical data of LIHC in The Cancer Genome Atlas(TCGA)were obtained from UALCA,and the data were grouped and processed.R3.2.2 software was used for analysis.The expression difference of BAP1 in tumor tissue and normal tissue was compared,and the difference of BAP1 expression in clinical data was analyzed.Life table method was used to calculate the survival rate.Kaplan-Meier method was used to compare the survival rate of patients with high and low BAP1 expression group,and the survival curve of patients was drawn.Results The median expression of BAP1 mRNA in LIHC tissues was 37.748 TPM,which was significantly higher than 18.444 TPM in normal tissues(P<0.01).The expression level of BAP1 in tumor tissues of patients with different gender,age,race,body mass,histological type,histological grade,lymph nodes,TNM stageⅠtoⅢ,and TP53 mutation was significantly different from that in normal tissues(P<0.05).There was no significant difference in the expression level of BAP1 between stageⅣtumor tissues and normal tissues(P>0.05).The median survival time was 27.18 months,and the 1-year,2-year,3-year,and 4-year survival rates were respectively 0.57%,0.31%,0.20%,0.13%,and 0.08%.The overall survival rate of patients with high expression of BAP1 mRNA was significantly lower than that in patients with medium and low expression of BAP1 mRNA(P<0.05).Conclusion BAP1 mRNA is highly expressed in LIHC,and the survival rate of patients with high expression of BAP1 mRNA is lower,suggesting poor prognosis.