根干反向生长模拟对厚朴皮总酚含量的诱导及代谢组学分析

Induction of total phenol content and metabolomics analysis of Magnolia officinalis errata bark by root trunk reverse growth simulation

以厚朴为试材,设置露晒根、包埋干根干反向生长模拟处理,以未作任何处理的根皮、干皮为对照,采用高效液相色谱法测定厚朴总酚,采用LC–MS技术分析差异代谢物。结果表明:包埋干、露晒根处理的干皮和根皮总酚含量均增加;厚朴中检测到873种代谢物,其中,对照的根皮组与干皮组显著性差异代谢物有107个,露晒根皮与对照干皮组显著性差异代谢物有71个,包埋干皮与对照根皮组显著性差异代谢物有76个,各处理组间差异代谢物以上调为主;差异代谢物主要包括脂质和类脂化合物、有机酸及衍生物、有机氧化物、木脂素、新木脂素及其相关化合物,且主要聚集在β–丙氨酸代谢、组氨酸代谢等途径;根干反向生长模拟处理能诱导处理部位与模拟部位之间差异代谢物数量显著降低,并引起氨基酸类物质和代谢途径积极响应。

Total phenols were determined by high performance liquid chromatography(HPLC)and the metabolomics were analyzed by LC-MS technique for the experimental material,Magnolia officinalis The studied sample did exposed root and embedded trunk treatments with the root bark and trunk bark as the control.The results showed that the total phenolic content of trunk bark and root bark increased in both the embedded trunk and exposed root treatments.107 of 873 detected metabolites in Magnolia officinalis,were significantly different between the control root bark and control trunk bark group;71 metabolites were significantly different between the exposed root bark and control trunk bark group,and 76 metabolites were significantly different between the embedded trunk bark and control root bark group.These differential metabolites among the treatment groups were mainly up-regulated.The simulated treatment of root trunk reverse growth caused significant differences in lipids and lipid compounds,organic acids and their derivatives,organic oxides,lignans,neolignans and their related compounds;and the differential metabolites were mainly clustered inβ-alanine metabolism,histidine metabolism and other pathways.The root trunk reverse growth simulation treatment induced a significant decrease in the amount of differential metabolites between the treated and simulated sites,and caused a positive response of amino acids and metabolic pathways.