纳米二氧化铈亚急性染毒对雄性小鼠脏器结构和氧化还原指标的影响

Subacute effect of CeO_(2)NPs on organ structures and redox indicators in male mice

目的 探究纳米二氧化铈(CeO_(2)NPs)亚急性腹腔染毒对ICR小鼠体重、主要脏器以及血清氧化还原指标的影响。方法 将雄性ICR小鼠35只随机分成5组,每组7只。设生理盐水对照组和不同浓度的CeO_(2)NPs(100、500、1000、5000μg/kg)试验组。小鼠腹腔隔日注射CeO_(2)NPs, 28 d后进行相关指标的检测。结果 与对照组相比,CeO_(2)NPs各剂量组小鼠体重增长差异不显著(P>0.05);5000μg/kg剂量组肝系数、附睾系数与精子存活率与对照组相比差异极显著(P<0.01)。5000μg/kg剂量组肝和脾内可见纳米氧化铈沉积,部分肝中可见肉芽肿。100μg/kg剂量组血清SOD和GSH-Px活力升高,MDA含量降低,体现出CeO_(2)NPs的抗氧化作用,随着注射剂量的升高,CeO_(2)NPs的氧化应激作用增强。结论 低剂量CeO_(2)NPs对机体较安全,并表现出一定的抗氧化作用,随着剂量增加,CeO_(2)NPs对机体的毒性也逐渐增强。

Objective To investigate the effects of CeO_(2)NPs subacute exposure on body weight,organ structures,and redox indicators in male mice.Methods Thirty-five male ICR mice were randomly divided into five groups with seven mice in each group.Normal control and CeO2NP(100,500,1000 and 5000μg/kg)groups were included.Mice were injected with CeO_(2)NPs intraperitoneally every other day for 28 days.Results Compared with the control group,no significant difference was found in weight gain(P>0.05),but significant differences were observed in the liver coefficient,epididymal coefficient,and sperm survival rate(P<0.01).In the 5000μg/kg group,CeO_(2)NPs were deposited in the liver and spleen,and granuloma was found SOD and GSH-Px activities in serum of the 100μg/kg group were increased,while MDA content was decreased,which indicated the antioxidant effect of CeO_(2)NPs.With the increase in dose,oxidative stress induced by CeO_(2)NPs was enhanced.Conclusions Low-dose-CeO_(2)NPs are safe and exert an antioxidant effect.With the increase in dose,the toxicity of CeO_(2)NPs also increases gradually.