IDentifier DNA分型盒(炎黄34)的法医学验证及应用评估

Forensic Validation and Application Evaluation of IDentifier DNA Typing Kit (Yan-Huang34)

目的 测试IDentifier DNA分型盒(炎黄34)的技术性能指标,评估其法医学应用价值。方法 根据《法庭科学人类荧光标记STR复合扩增检测试剂质量基本要求》(GB/T 37226—2018),从种属特异性、分型准确性、灵敏度、适应性、耐受性、一致性、均衡性、反应条件验证、混合样本、稳定性、批间差11个方面对IDentifier DNA分型盒(炎黄34)进行测试。比较IDentifier DNA分型盒(炎黄34)与Power Plex~?Fusion 6C系统、Versa Plex~?27PY系统、Veri Filer~(TM) Plus PCR扩增试剂盒的系统效能。使用IDentifier DNA分型盒(炎黄34)检测日常案件中的拭子类生物检材,观察其STR检验结果 。结果 IDentifier DNA分型盒(炎黄34)具有良好的种属特异性、分型准确性、适应性、耐受性和均衡性,灵敏度可达0.062 5 ng,能检测案件中不同类型的检材、降解检材及混有抑制剂的检材,对混合比例为4∶1以内的样本均能获得完整分型。该试剂盒的系统效能非常高,TDP可达1-1.08×10~(-37),CPE_(trio)和CPE_(duo)分别可达1-5.47×10~(-14)和1-6.43×10~(-9)。针对案件中的接触类生物检材,其有效检出率可达21.05%。针对亲缘关系鉴定,单亲鉴定和全同胞鉴定的系统效能均得到了有效提高。结论 IDentifier DNA分型盒(炎黄34)的上述性能指标符合要求,可用于个体识别及亲权鉴定,适合在法庭科学领域应用。

Objective To investigate the technical performance of IDentifier DNA typing kit(YanHuang34)and evaluate its forensic application value.Methods Following the Criterion of Forensic Science Hu‐man Fluorescence STR Multiplex Amplification Reagent(GB/T 37226—2018),IDentifier DNA typing kit(YanHuang34)was verified in 11 aspects of species specificity,veracity,sensibility,adaptability,in‐hibitor tolerance,consistency,balance,reaction condition verification,mixed samples,stability and inter batch consistency.The system efficiency of IDentifier DNA typing kit(YanHuang34)was compared with the PowerPlex®Fusion 6C System,VersaPlex®27PY System and VeriFilerTM Plus PCR Amplifica‐tion Kit.The IDentifier DNA typing kit(YanHuang34)was used to detect the swabs of biological samples in daily cases and the STR performances were observed.Results IDentifier DNA typing kit(YanHuang34)had good species specificity,veracity,adaptability,inhibitor tolerance and balance.The sensibility was up to 0.0625 ng.It was able to detect different types of samples,degraded samples and inhibitor mixed samples.Complete DNA typing could be obtained for samples with the mixture ratio less than 4∶1.The system efficiency of IDentifier DNA typing kit(YanHuang34)was very high,with TDP up to 1-1.08×10-37,CPEtrio and CPEduo up to 1-5.47×10-14 and 1-6.43×10-9,respectively.For the touched biological samples in actual cases,the effective detection rate was 21.05%.The system efficiency of kinship,single parent and full sibling identifications was effectively improved.Conclusion The IDentifier DNA typing kit(YanHuang34)is adaptive to the GB/T 37226—2018 requirements.It can be used for individual identification and paternity identification,and is suitable for application in the field of forensic science.