饲料中添加抗菌肽对肉牛免疫机能的影响

Effects of adding antimicrobial peptide to feeds on the immune function of beef cattle

[目的]为抗菌肽在兽医临床上的使用剂量和使用方法提供理论参考。[方法]采用随机数字表法将80头6月龄的西门塔尔牛分为4组,每组20头,分别为对照组、抗菌肽低剂量组、中剂量组、高剂量组。在试验第60天采集西门塔尔牛的静脉血,并应用酶标仪检测西门塔尔牛血清中IL-6、IL-10、TNF-α、IgG、IgA和IgM含量。[结果]在试验第60天,与对照组相比,抗菌肽低剂量组、中剂量组和高剂量组西门塔尔牛血清中IL-10含量分别显著提高30.33%、51.36%、60.85%,IgA含量分别显著提高15.94%、36.23%、43.48%;抗菌肽中剂量组、高剂量组西门塔尔牛血清中IgG含量分别显著提高45.03%、51.27%;各组血清中IgM含量无显著差异。[结论]抗菌肽能够一定程度提升肉牛机体的体液免疫和细胞免疫功能。

[Objectives]To provide theoretical reference for the dosage and usage of antimicrobial peptides in veterinary clinic.[Methods]80 Simmental cattle aged six months were divided into 4 groups using a random number table method,with 20 cattle in each group,namely the control group,group with low-dose of antimicrobial peptide,group with medium dose of antimicrobial peptide,and group with high-dose of antimicrobial peptide.The venous blood from Simmental cattle was collected on the 60th day of the experiment,and the enzyme-linked immunosorbent assay(ELISA)was used to detect the content of IL-6,IL-10,and TNF-α,IL-10,IgG、IgA and IgM in serum from Simmental cattle.[Results]The content of IL-10 in serum collected from Simmental cattle on the 60th day of the experiment was significantly increased by 30.33%,51.36%,and 60.85%in the low-dose,medium dose,and high-dose groups of antimicrobial peptides,respectively,compared to the control group.The content of IgA was significantly increased by 15.94%,36.23%,and 43.48%,respectively.The content of IgG in Simmental bovine serum of medium dose group and high dose group was significantly increased by 45.03%and 51.27%,respectively.There was no significant difference in content of IgM in the serum of all groups.[Conclusions]Antibacterial peptides can enhance the humoral and cellular immunity of beef cattle to a certain extent.