有氧运动联合二甲双胍改善2型糖尿病大鼠胰岛素抵抗及其对血清内脂素的影响

Effects of Aerobic Exercise Combined with Metformin on Insulin Resistance in Type 2 Diabetic Rats and Its Effect on Serum Visfatin

目的:本研究旨在通过观察6周有氧运动、二甲双胍及其两者联合干预,对2型糖尿病(T2DM)大鼠胰岛素抵抗的改善作用,及对血清内脂素(Visfatin)的影响,为有氧运动联合二甲双胍方案在2型糖尿病的治疗中的效果,提供试验依据。方法:选用8周龄SD雄性健康大鼠共计50只,随机挑选10只大鼠为正常对照组(C组),用标准普通饲料进行喂养。剩余的40只大鼠进行T2DM动物模型塑造。造模成功后的大鼠随机分为:糖尿病对照组(DM)、糖尿病运动组(DME)、糖尿病药物组(DMM)、糖尿病药物+运动组(DMME),进行6周的干预。6周后,检测各组大鼠TG、TC、FBG、FINS和Visfatin的含量。结果:(1)与C组相比,DM组TC和TG水平均表现出极显著升高(P<0.01);与DM组相比,DME组、DMM组、DMME组TC水平均显著性降低(P<0.05);与DM组相比,DME组、DMM组、DMME组TG含量均呈下降趋势,其中DMM组具有极显著性差异(P<0.01)。(2)与C组相比,DM组胰岛素抵抗指数(HOMA-IR)显著升高(P<0.05);与DM组相比,DME组、DMM组、DMME组胰岛素抵抗指数均呈下降趋势,其中以DME组、DMME组下降显著(P<0.05)。(3)与C组相比,DM组Visfatin水平极显著升高(P<0.01);与DM组相比,DME组、DMM组、DMME组Visfatin水平均呈下降趋势,其中以DMME组下降趋势最为明显,但无显著性差异(P>0.05)。结论:(1)有氧运动、二甲双胍及二者联合均能在一定程度上纠正T2DM大鼠糖脂代谢的紊乱,改善T2DM大鼠胰岛素抵抗。(2)有氧运动、二甲双胍二者联合干预的效果有优于单一干预的趋势。

Objective:The aim of this study was to observe the effects of 6 weeks aerobic exercise,metformin and their combined intervention on insulin resistance in type 2 diabetes mellitus(T2DM)rats and the effect of serum Visfatin,so as to provide experimental evidence for the effect of aerobic exercise combined with metformin in the treatment of type 2 diabetes.Methods:A total of 508-week-old SD male healthy rats were selected,and 10 rats were randomly selected as the normal control group(Group C).The remaining 40 rats were modeled as T2DM animal model.Rats after successful modeling were randomly divided into diabetic control group(DM),diabetic exercise group(DME),diabetic drug group(DMM),diabetic drug+exercise group(DMME)for 6 weeks'intervention.Afer 6 weeks,the contents of TG,TC,FBG,FINS and Visfatin were measured.Results:(1)Compared with group C,the levels of TC and TG in DM group increased significantly(P<0.01);Compared with DM group,TC levels in DME group,DMM group and DMME group decreased significantly(P<0.05);Compared with DM group,TG content in DME group,DMM group and DMME group decreased,and there was a very significant difference in DMM group(P<0.01).(2)Compared with group C,HOMA-IR in DM group was significantly higher(P<0.05);Compared with DM group,the HOMA-IR indexes of DME group,DMM group and DMEM group showed a downward trend.Among them,DME group and DMEM group decreased significantly(P<0.05).(3)Compared with group C,the expression of Visfatin in DM group was significantly higher(P<0.01);Compared with DM group,the Visfatin levels in DME group,DMM group and DMEM group showed a downward trend,especially in DMEM group,but there was no significant difference(P>0.05).Conclusions:(1)Aerobic exercise,metformin and their combination can correct the disorder of glucose and lipid metabolism and improve insulin resistance in T2DM rats to a certain extent.(2)The effect of combined intervention of aerobic exercise and metformin is better than that of single intervention.