应用16S rRNA基因序列鉴定15只鸟类残体

Identification of 15 bird remains using the 16S rRNA gene sequence

目的 应用鸟类线粒体16S rRNA基因PCR测序方法进行涉案鸟类残体的物种鉴定。方法 以15只未知鸟类残体冷冻肌肉组织为研究材料,采用PCR扩增DNA条形码片段16S rRNA。结果 通过序列在线比对和系统发育分析,判定15份检材涉及珠颈斑鸠(Spilopelia chinensis)、斑鸫(Turdus eunomus)、麻雀(Passer montanus)、金翅雀(Chloris sinica)、燕雀(Fringilla montifringilla)和北红尾鸲(Phoenicurus auroreus),共计6种鸟类物种,隶属2目6科6属,均为“三有名录”保护动物。结论 16s rRNA基因片段可以作为一个可靠手段对鸟类残体进行准确的物种鉴定,为司法案件的定性与量刑提供可靠的证据。

Objective The mitochondrial 16S rRNA gene PCR sequencing method was applied to identify the bird species involved in the case of bird remains.Methods Using frozen muscle tissue samples from 15 unknown bird remains,the PCR amplification of the 16S rRNA DNA barcode fragment was performed.Results Through sequence alignment and phylogenetic analysis,it was determined that the 15 samples were associated with six bird species,including four Streptopelia chinensis,one Turdus eunomus,five Passer montanus,two Chloris sinica,two Fringilla montifringilla,and one Phoenicurus auroreus.These species belong to 2 orders,6 families,and 6 genera,all of which are protected as listed species under the wildlife conservation regulations.Conclusion The 16S rRNA gene segment can be regarded as a reliable approach for accurately identifying bird species from remains,providing a dependable basis for qualitative and sentencing determinations in judicial cases.