胶束毛细管电泳同时分离测定葛根及其制剂中的5种异黄酮

Simultaneous separation and determination of five isoflavones in Paeonia Lactiflora Radix and its preparations by micellar capillary electrophoresis

建立了一种新的胶束毛细管电泳方法,用于同时分离检测葛根素、大豆苷、3’-甲氧基葛根素、3’-羟基葛根素和4’-甲氧基葛根素5种异黄酮。优化的实验条件为:以40 mmol/LNa_(2)B_(4)O_(7)+4 mmol/L NaOH(pH 9.62)为运行缓冲溶液,5%(V/V)甲醇和14 mmol/L的十二烷基磺酸钠(SDS)为添加剂,分离电压22 kV,检测波长250 nm,进样时间5 s。在优化条件下,葛根素、大豆苷、3’-甲氧基葛根素、3’-羟基葛根素和4’-甲氧基葛根素5种异黄酮可在14 min内完成分离检测,各目标组分的峰面积与其浓度之间的线性关系良好。该方法用于葛根及其制剂中5种异黄酮的定量分析,加标回收率范围为95.6%~104.8%,相对标准偏差不超过4.0%。

A new method of micellar capillary electrophoresis was developed for the simultaneous separation and detection of puerarin,daidzin,3'-methoxypuerarin,3'-hydroxypuerarin,and 4'-methoxypuerarin.The buffer solution consisted of 40 mmol/L Na_(2)B_(4)O_(7)and 4 mmol/L NaOH(pH 9.62),with 5%(V/V)methanol and 14 mmol/L sodium dodecyl sulfate(SDS)as additives.The separation voltage was 22 kV,the detection wavelength was 250 nm,and the injection time was 5 s.Under the optimized conditions,five isoflavones could be separated and detected within 14 min,and the linear relationship between the peak area of each target component and its concentration was good.The method was applied to the quantitative analysis of five isoflavones in Pueraria lobata and its preparations.The spiked recoveries ranged from 95.6%to 104.8%,and the relative standard deviation was not more than 4.0%.