气相色谱-质谱法检测葡萄酒中二甲基二碳酸盐

Determination of dimethyldicarbonate in wine by gas chromatography-mass spectrometry

建立了气相色谱-质谱(GC-MS)法检测葡萄酒中二甲基二碳酸盐代谢产物碳酸甲乙酯的方法,进而建立了二甲基二碳酸盐添加量的检测方法。优化了仪器参数、色谱条件、萃取试剂种类和体积、提取时间等参数。结果表明,方法的线性范围为0.5~30 mg/L,在红葡萄酒、甜白葡萄酒、起泡葡萄酒中添加0.5,10.0,30.0 mg/L的碳酸甲乙酯,方法回收率为90.0%~110%,相对标准偏差为3.6%~6.7%,检出限为0.25 mg/L,定量限为0.5 mg/L。该方法适合于葡萄酒中碳酸甲乙酯以及二甲基二碳酸盐的检测。

A method for the determination of methyl ethyl carbonate a metabolite of dimethyl decarbonate in wine by gas chromatography-mass spectrometry(GC-MS)was developed,and then a method for the determination of the added amount of dimethyldecarbonate was established.The analytical equipment parameters,chromatographic conditions,type and volume of extraction reagents,and extraction time were optimized.The linear range of this method was 0.5-30 mg/L.The average recoveries for methyl ethyl carbonate spiked at 0.5,10.0 and 30.0 mg/L in red wine,sweet white wine and sparkling wine ranged from 90.0%to 110%,the relative standard deviations were 3.6%-6.7%,the limit of detection was 0.25 mg/L,and the limit of quantification was 0.5 mg/L.The method is suitable for the detection of methyl ethyl carbonate and dimethyl dicarbonate in wine.