基于久洛尼定的铝离子荧光探针的合成与性能

Synthesis and properties of fluorescent probes based on julolidine for the detection of Al^(3+)

设计合成了基于久洛尼定的酰腙荧光探针JNPH,并使用核磁共振波谱(NMR)和高分辨质谱(HRMS)表征了JNPH的结构。采用荧光发射光谱研究了在DMSO/Tris-HCl(7∶3,V∶V)缓冲体系中探针JNPH对金属离子的识别能力。结果表明,在DMSO缓冲液中探针JNPH对Al^(3+)显示出选择性荧光增强识别,且具有良好的识别灵敏性。Job曲线显示探针JNPH与Al^(3+)结合的化学计量比为1∶1。探针JNPH对Al^(3+)的识别不受大多数金属离子的干扰,检测Al^(3+)的线性范围为0~80μmol/L,检出限为0.38μmol/L。探针JNPH具有快速响应识别Al^(3+)的能力,响应时间为60 s。利用^(1)H-NMR研究了探针JNPH对Al^(3+)的识别机理,并结合Job曲线推测了探针JNPH与Al^(3+)的作用模式。

An acylhydrazone-based fluorescent probe JNPH derived from julonidine was designed and synthesized,and its structure was characterized by nuclear magnetic resonance(NMR)and high resolution mass spectrometry(HRMS).The recognition ability of probe JNPH for metal ions in DMSO/Tris-HCl(7∶3,V∶V)buffer system was investigated by fluorescence emission spectroscopy.The results showed that the probe JNPH had an excellent selective and sensitivity recognition for Al^(3+)with fluorescence enhancement in DMSO buffer solution.Job's curve showed that the stoichiometric ratio between probe JNPH and Al^(3+)was 1∶1.The recognition of Al^(3+)by probe JNPH was not interfered by most metal ions.The linear detection range of probe JNPH for Al^(3+)was 0-80μmol/L,and the detection limit was 0.38μmol/L.Probe JNPH had the ability for rapid response to identify Al^(3+)in 60 s.The sensing mechanism was studied by^(1)H-NMR spectroscopy,and the binding mode between probe JNPH and Al^(3+)was inferred by combining^(1)H-NMR with Job's curve.