时间分辨荧光免疫层析法检测青海牦牛肉中磺胺类药物残留

Detection of sulfonamides residues by time-resolved fluorescence immunochromatography in yak meat

以羧基化铕微球作为荧光标记物,将磺胺类药物抗原和羊抗鼠免疫球蛋白分别包被于硝酸纤维素(NC)膜上得到检测线T和控制线C,制备了免疫层析试纸条。依据T线与C线荧光信号值的比值(T/C)以及样品中磺胺类药物的浓度含量建立定量标准曲线。结果表明,该方法检测的牦牛肉定量限均低于2μg/kg,样品的添加回收率在90%~115%,其中批内离散系数均低于10%、批间离散系数均低于13%。通过对盲样的测定,本法结果和仪器法结果符合率为100%。

Using carboxylated europium microspheres as fluorescent markers,sulfamethazine antigen and goat anti-mouse immunoglobulin were coated on nitrocellulose(NC)membrane,which were marked as detection line T and control line C,respectively,to make immunochromatographic strips.According to the ratio of the fluorescence signal value of T line and C line(T/C)and the concentration of sulfonamides in the sample,a quantitative standard curve was established.The results showed that the limit of quantification of yak meat detected by this method was less than 2μg/kg,and the recoveries of all samples were between 90%and 115%.The intra-assay relative standard deviations(RSDs)were less than 10%and the inter-assay RSDs were less than 13%.Through the determination of blind samples,the consistence rate between this method and the instrument method was 100%.