基于氮掺杂碳量子点荧光探针检测谷胱甘肽

Detection of glutathione based on nitrogen-doped carbon dots as a fluorescence probe

以水杨酸和乙二胺为原料,采用一步水热法合成了绿色荧光的氮掺杂碳量子点(N-CDs)。采用透射电子显微镜(TEM)、红外吸收光谱(FT-IR)、荧光光谱等对N-CDs进行了表征。由于从N-CDs到MnO_(2)纳米片的荧光共振能量转移(FRET),N-CDs的荧光可以被MnO_(2)纳米片猝灭。然而,谷胱甘肽(GSH)的存在会使MnO_(2)纳米片还原为Mn^(2+),从而导致荧光信号的充分恢复。基于上述原理,建立了一种检测GSH的方法,其线性范围为0.1~130μmol/L,检出限为0.089μmol/L。此外,N-CDs显示出低细胞毒性,已成功用于人肝癌细胞(HepG2)中的GSH成像。

The green fluorescent nitrogen-doped carbon quantum dots(N-CDs)were synthesized by one-step hydrothermal method using salicylic acid and ethylenediamine as raw materials.The synthesized N-CDs were characterized by transmission electron microscopy(TEM),Fourier transform infrared spectroscopy(FT-IR),and fluorescence spectroscopy.Due to the fluorescence resonance energy transfer(FRET)from N-CDs to the MnO_(2)nanosheets,the fluorescence of N-CDs can be quenched by MnO_(2)nanosheets.However,the presence of glutathione(GSH)would reduce MnO_(2)nanosheets to Mn^(2+),resulting in sufficient recovery of fluorescent signal.Based on the above principle,a method was established to detect GSH with the linear range of 0.1-130μmol/L and the limit of detection of 0.089μmol/L.In addition,the N-CDs displayed low cytotoxicity and had been successfully used to image GSH in HepG2 cells.