建立一种基于BAS化学发光技术检测血清β-hCG,Prog水平的抗生物素干扰方法

Establish an Anti-biotin Interference Method to Detect Serumβ-hCG and Prog Levels Based on BAS Immunoassays

目的在基于生物素-链霉亲和素(biotin-avidin/streptavidin,BAS)的化学发光技术检测人绒毛膜促性腺激素β亚基(β-human chorionic gonadotropin,β-hCG)和孕酮(progesterone,Prog)时,建立一种简易、有效的抗生物素干扰方法。方法采用两种浓度链霉亲和素磁珠(streptavidin coated magnetic micro particles,M)检测不同生物素浓度的高、中、低水平的β-hCG和Prog血清,通过回收试验评价两种浓度M的抗生物素干扰能力以及采用低浓度M的校准曲线时高浓度M检测的准确度。结果①β-hCG和Prog的抗生物素干扰能力在低浓度M(0.72 mg/ml)时分别为100和25 ng/ml,在高浓度M(1.44 mg/ml)时分别为500和50 ng/ml。②使用和低浓度M相同的校准曲线时,高浓度M对于生物素浓度在500 ng/ml以下的β-hCG三个水平的回收率均在90%~110%之间;对于生物素浓度在50 ng/ml以下的高、中水平的Prog,其回收率在90%~110%之间。结论在基于BAS化学发光技术检测血清β-hCG和Prog时,采用高浓度M(1.44 mg/ml)是一种简易、有效且可靠的抗生物素干扰方法。

Objective A simple and effective anti-biotin interference method was established to detect human chorionic gonadotropinβsubunit(β-hCG)and progesterone(Prog)in BAS immunoassays.Methods Using two different concentrations of streptavidin magnetic particles(M)to detect high,medium and low levels ofβ-hCG and Prog serum with different biotin concentrations,the anti-biotin interference ability of two kinds of M and the accuracy of high concentration M to detectβ-hCG and Prog were evaluated through recovery test when the calibration curve of low concentration M is adopted.Results①The anti-biotin interference ability ofβ-hCG and Prog were 100 and 25 ng/ml respectively at low concentration M(0.72 mg/ml),and were 500 and 50 ng/ml respectively at high concentration M(1.44 mg/ml).②When using the same calibration curve as low concentration M,the recovery rate of high concentration M forβ-hCG at three levels with biotin below 500 ng/ml were between 90%and 110%,for Prog with high and medium levels of biotin below 50 ng/ml,the recovery rate were between 90%~110%.Conclusion When detecting serum nterference ability ofβ-hCG mmunoassays,the method of high concentration M(1.44 mg/mL)is a simple,effective and reliable anti-biotin interference program.