香豆素类光敏基团对脱氧核苷5′-羟基的选择性保护

Selective Protection of 5′-Hydroxyl Group of Deoxynucleoside by Photosensitive Groups of Coumarin

以香豆素类化合物作为光可降解保护基,探究了香豆素类化合物对脱氧核苷中5′-位羟基的保护和脱保护性能,为在传统核酸合成中使用光脱保护替代酸脱保护做了前期工作。选择可在紫外及蓝光波段区间进行光化学反应的7-二乙胺基-4-羟甲基香豆素类化合物作为保护基团,并以碳酸酯的形式连接在N-4-乙酰基脱氧胞苷的5′-羟基上,考察该化合物在紫外和蓝光光照下的光化学性质,为构建光调节DNA合成进行前期研究并奠定良好基础。

In this paper,we utilized the photolabile coumarin compounds to replaced the acid removable protective groups on the 5′-hydroxyl group on the deoxyriboside in nucleic acids’synthesis.The violent and blue light responsive coumarin segments were attached on the 5′-hydroxyl group on the N-4 acetyl deoxycytidine via the carbonate linker,and the synthesis photolysis was investigated under different light irradiation.