盆腔炎性疾病患者血清miR-140-5p、TLR4的表达水平及其临床意义

Serum miR-140-5p and TLR4 levels in patients with pelvic inflammatorydisease and their clinical significance

目的:探讨盆腔炎性疾病(PID)患者血清中miR-140-5p、Toll样受体4(TLR4)的表达水平及其临床意义,旨在为诊断和治疗PID患者提供相关依据。方法:选取2020年10月至2021年10月本院收治的132例PID患者为PID组,100例体检健康人群作为对照组;采用实时荧光定量PCR(RT-qPCR)技术检测miR-140-5p、TLR4表达水平;采用酶联免疫吸附法(ELISA)检测TNF-α、IL-6、IL-4和IL-1β的含量;Pearson法分析miR-140-5p、TLR4的相关性以及二者与TNF-α、IL-6、IL-4、IL-1β的相关性;绘制ROC曲线分析miR-140-5p、TLR4对PID的诊断价值。结果:PID组血清miR-140-5p表达水平显著低于对照组,TLR4的表达水平显著高于对照组(P<0.05),二者表达水平具有负相关关系(P<0.05);随着PID严重程度增加miR-140-5p的表达水平降低,TLR4的表达水平升高(P<0.05);PID组患者血清TNF-α、IL-6、IL-1β水平较对照组显著升高,IL-4水平显著降低(P<0.05);miR-140-5p与TNF-α、IL-6、IL-1β水平均呈负相关,与IL-4水平呈正相关(P<0.05);TLR4与TNF-α、IL-6、IL-1β水平均呈正相关,与IL-4水平呈负相关(P<0.05);二者联合诊断的AUC高于miR-140-5p、TLR4单独诊断的AUC(Z=2.214,P<0.001;Z=2.012,P=0.031)。结论:PID患者血清中miR-140-5p表达水平显著降低,TLR4表达水平显著升高,二者联合对PID具有一定的诊断价值。

Objective:To investigate the expression level of miR-140-5p and Toll-like receptor 4(TLR4)in serum of patients with pelvic inflammatory disease(PID)and their clinical significance,in order to provide relevant basis for diagnosis and treatment of patients with PID.Methods:From October 2020 to October 2021,132 PID patients admitted to our hospital were included in the PID group,and 100 healthy people with physical examination were included in the control group;real-time quantitative PCR(RT-qPCR)technology was applied to detect the expression levels of miR-140-5p and TLR4;enzyme-linked immunosorbent assay(ELISA)was applied to detect the levels of TNF-α,IL-6,IL-4 and IL-1β;Pearson method was applied to analyze the correlation between miR-140-5p and TLR4 and their correlation with TNF-α,IL-6,IL-4 and IL-1β;ROC curve was drawn to analyze the diagnostic value of miR-140-5p and TLR4 for PID.Results:The expression level of serum miR-140-5p in the PID group was greatly lower than that in the control group,and the expression level of TLR4 was greatly higher than that in the control group(P<0.05),and the two expression levels were negatively correlated(P<0.05);with the increase of PID severity,the expression level of miR-140-5p decreased,and the expression level of TLR4 increased(P<0.05);the serum levels of TNF-α,IL-6 and IL-1βin the PID group were greatly higher than those in the control group,and the level of IL-4 was greatly lower(P<0.05);miR-140-5p was negatively correlated with the levels of TNF-α,IL-6 and IL-1β,and positively correlated with the level of IL-4(P<0.05);TLR4 was positively correlated with the levels of TNF-α,IL-6,IL-1β,and negatively correlated with the level of IL-4(P<0.05);the AUC of the combined diagnosis was higher than that of miR-140-5p and TLR4 alone(Z=2.214,P<0.001;Z=2.012,P=0.031).Conclusion:The expression level of miR-140-5p in serum of PID patients was greatly decreased,and the expression level of TLR4 was greatly increased.The combination of the two has certain diagnostic value for PID.