常温下三卡因和丁香酚对条纹小鲃的麻醉效果研究

Anesthetic Efficacy of Tricaine and Eugenol on Barbel Barbodes semifasciolatus at Room Temperature

为提高条纹小鲃在常温下养殖生产操作及运输中的存活率,将野外采集的条纹小鲃经循环水暂养驯化14 d,在水温(25.0±0.5)℃下,开展三卡因和丁香酚对体质量(0.28±0.09)g条纹小鲃的有效麻醉试验:分别经质量浓度为140 mg/L的三卡因、质量浓度为25 mg/L的丁香酚深度麻醉后的离水暴露试验,分别经质量浓度为10 mg/L的三卡因、质量浓度为8 mg/L的丁香酚镇静后的模拟运输试验,探讨常温下不同操作目的2种麻醉剂的应用效果。试验结果显示,三卡因和丁香酚麻醉条纹小鲃的有效质量浓度分别为100~140 mg/L、20~30 mg/L。经质量浓度140 mg/L三卡因、质量浓度25 mg/L丁香酚深度麻醉的条纹小鲃,在空气中分别暴露6、8 min内复苏率为100%。选择三卡因质量浓度10 mg/L、丁香酚质量浓度8 mg/L模拟运输,48 h内均以鱼水质量比1∶15效果最佳;如运输72 h,可使用三卡因麻醉,以鱼水质量比1∶20效果最佳,存活率达90.74%。试验结果表明,三卡因和丁香酚均对条纹小鲃有良好的麻醉效果,在实际应用中应根据不同的操作目的并结合其他要素,选用合适的麻醉质量浓度。

In order to improve the survival rate in operation and transportation,the anesthetic effect of tricaine and eugenol on barbel Barbodes semifasciolatus with body weight of(0.28±0.09)g acclimated for 2 weeks was investigated under water temperature of(25.0±0.5)℃:the barbel were exposured to 80,90,100,110,120,130,140,150,160 mg/L tricaine and 15,20,25,30 and 35 mg/L eugenol to explore the effective anesthetic dose;the barbel were exposed to tricaine with concentration of 140 mg/L and eugenol with a mass concentration of 25 mg/L after deep anesthesia exposure to water for 0,2,4,6,8 and 10 min,and then put back into clean water for recovery,recording the recovery time and counting the recovery rate.After exposing the barbel to tricaine with concentration of 10 mg/L and eugenol with concentration of 8 mg/L,the transport test was simulated to explore the application effect of two anesthetics for different operating purposes at room temperature.The results showed that the suitable mass concentrations were 100-140 mg/L for tricaine and 20-30 mg/L for eugenolm.After the deep anesthesia at tricaine mass concentration of 140 mg/L,the suitable exposure time in air was below 6 min,while the barbel was recoveryed to 100%within 8 min of air exposure after deep anesthesia at eugenol mass concentration of 25 mg/L.An anesthesia transport test showed the two anesthetics both had good transport effect within 48 h at fish:water ratio of 1∶15,and tricaine was available at fish:water ratio of 1∶20 under 72 h transportation with the survival rate up to 90.74%.Both tricaine and eugenol had good anaesthetic effects on the barbel in practical applications,were chosen for a suitable anaesthetic mass concentration according to different operating purposes and in combination with other elements.