川芎嗪对小鼠生长性能、血液生理、生化指标及抗氧化功能的影响

Effects of Tetramethyl Pyrazine on Growth Performance,Blood Physiology,Biochemical Indexes and Antioxidant Function of Mice

试验旨在探究川芎嗪对小鼠生长性能、脏器指数、血液生理指标及抗氧化功能的影响。试验将72只健康雄性小鼠随机分为6组,每组12只,分别为空白(K)组、试验1、2、3、4、5组,适应性喂养7 d。空白组小鼠灌服0.2 mL/10 g体重的0.8%羧甲基纤维素钠溶液,试验1、2、3、4、5组分别灌服50、100、150、200、250 mg/kg体重的川芎嗪,连续灌胃14 d。最后一次灌服川芎嗪溶液24 h后,小鼠眼静脉丛取血,随后处死,并采集心脏、肝脏、脾脏、肺脏、肾脏,称量各器官质量,计算小鼠脏器指数;检测血液生理指标;测定血清中天冬氨酸氨基转移酶(AST)、谷氨酸-丙酮酸转氨酶(ALT)的活性,测定血清和肝组织中生化指标还原型谷胱甘肽(GSH)、丙二醛(MDA)、总超氧化物歧化酶(TSOD)、总抗氧化能力(T-AOC)。结果表明,各试验组和空白组小鼠试验末体重和脏器指数均无显著差异(P>0.05);与空白组相比,试验1、2、3、4组的白细胞数目、淋巴细胞数目有显著性差异(P<0.05),试验1、2、4组的淋巴细胞百分比有显著性差异(P<0.05),试验1、2、4、5组的血小板数目有显著性差异(P<0.05);与空白组相比,试验3、4组能够显著降低小鼠血清ALT活性(P<0.05);与空白组相比,试验3、4组可显著升高血清中GSH含量(P<0.05),试验2、3、4、5组可显著升高血清中T-SOD含量(P<0.05),试验3、4、5组可显著升高血清中T-AOC含量(P<0.05),试验2、3、4、5组可降低升高血清中MDA含量(P<0.05)。与空白组相比,试验1、2、3、4组可显著升高肝组织中GSH、T-SOD含量(P<0.05);试验3组可显著降低MDA含量(P<0.05)。由此可见,川芎嗪可显著提高机体的抗凝血能力和抗氧化能力,给药浓度在150 mg/kg时提升抗氧化能力效果最显著。

The experiment aimed to investigate the effects of tetramethyl pyrazine on the growth performance,organ index,blood physiological index and antioxidant function of mice.Seventy-two healthy male mice were randomly divided into 6 groups of 12 mice each,namely blank(K)group,test group 1,group 2,group 3,grouP 4 and group 5,and acclimatized for 5 d.Mice in the blank group were given 0.2 mL/10 g body weight of 0.8% sodium carboxymethylcellulose solution,and the mice in groups 1,2,3,4 and 5 were gavaged with 50,100,150,200 mg/kg and 250 mg/kg body weight of tetramethyl pyrazine for 14 d.After the last gavage of tetramethyl pyrazine solution for 24 h,blood was taken from the ophthalmic vein plexus of the mice and subsequently executed,and the heart,liver,spleen,lungs and kidneys were collected.The organs were weighed and the organ indices were calculated.Blood physiological parameters were measured;the activities of aspartate aminotransferase(AST)and alanine transaminase(ALT)in serum were measured,and the biochemical parameters of reduced glutathione(GSH),malondialdehyde(MDA),total superoxide dismutase(T-SOD),total antioxidant capacity(T-AOC).The results showed that there was no significant difference in body weight and organ index at the end of the test between each test group and the blank group of mice(P>0.05);compared with the blank group,there were significant differences in the number of leukocytes and lymphocytes in test groups 1,2,3 and 4(P<0.05),and the percentage of lymphocytes in test groups 1,2 and 4(P<0.05);compared with the blank group,the compared with the blank group,test groups 3 and 4 could significantly reduce the serum ALT activity of mice(P<0.05);compared with the blank group,test groups 3 and 4 could significantly increase the serum GSH content(P<0.05),test groups 2,3,4 and 5 could significantly increase the serum T-SOD content(P<0.05),test groups 3,4 and 5 could significantly increase the serum T-AOC content(P<0.05),and the test groups 2,3,4 and 5 reduced the increase of MDA in serum(P<0.05).Compared with the blank group,test groups 1,2,3 and 4 significantly increased GSH and T-SOD levels in liver tissues(P<0.05);test group 3 significantly decreased MDA levels(P<0.05).This shows that tetramethyl pyrazine can significantly improve the anti-coagulant ability and antioxidant capacity of mice,with the most significant effect when administered at 150 mg/kg.