5-HMF高效液相色谱检测方法的建立及功能糖与不同产地党参、何首乌和熟地中5-HMF含量分析

Establishment of a high performance liquid chromatographic method for the determination of 5-HMF and analysis of 5-HMF content between functional sugar and Radix Codonopsis Pilosulae,Radix Polygonui Multiflori and Radix Rehmanniae Preparata from different origins

本试验旨在建立5-羟甲基糠醛(5-hydroxymethylfurfural,5-HMF)的高效液相色谱检测方法,检测不同产地党参、制首乌、熟地黄以及5个不同批次功能糖中5-HMF含量。采用C_(18)(4.6 mm×250.0 mm,5μm)色谱柱分离,流动相为甲醇-水(8∶92),流速为1.0 mL/min,柱温为30℃,进样量为10μL,紫外检测器,检测波长为280 nm。经方法学考察,显示该方法线性关系良好;精密度试验测定峰面积RSD平均值为1.438%;重复性试验显示HPLC重复性良好;稳定性试验峰面积RSD范围为1.338%~7.307%;加标回收率显示良好。中药及功能糖5-HMF含量检测结果显示,党参、制首乌和熟地黄相比,熟地黄中5-HMF含量较高,党参中5-HMF含量较低,其中又以河北产熟地黄中5-HMF含量最高。不同批次功能糖中5-HMF含量无显著性差异,但其含量是河北产熟地黄的90~100倍。通过现代化技术,用功能糖成功复制了中药中的活性成分5-HMF,降低了其生产成本,具有良好的发展前景。

The aim of this experiment was to develop a high performance liquid chromatographic method for the determination of 5-hydroxymethylfurfural(5-HMF)in different origins of Radix Codonopsis Pilosulae,Radix Polygonui Multiflori,Radix Rehmanniae Preparata and five batches of functional sugars.The separation was performed on a C_(18)(4.6 mm×250.0 mm,5μm)column with a mobile phase of methanol-water(8∶92)at flow rate of 1.0 mL/min,column temperature of 30℃,sample volume of 10μL and UV detector at 280 nm.The mean RSD of the peak area was 1.438%in the precision test;the reproducibility test showed good repeatability by HPLC;the RSD of the peak area ranged from 1.338%to 7.307%in the stability test;the spiked recovery showed good results.5-HMF content determination showed that Radix Rehmanniae Preparata had the higher 5-HMF content,while Radix Codonopsis Pilosulae had the lower 5-HMF content,among which Radix Rehmanniae Preparata from Hebei had the highest 5-HMF content.There was no significant difference in the 5-HMF content between different batches of functional sugars,but its content was 90-100 times higher than that of Radix Rehmanniae Preparata from Hebei.Through modern technology,functional sugars has successfully replicated the active ingredient 5-HMF in traditional Chinese medicine,greatly reducing the cost of its application in the breeding industry,which has good development prospects.