RP-HPLC法同时测定茵栀解毒颗粒中绿原酸和栀子苷的含量

Simultaneous determination of chlorogenic acid and geniposide in Yinzhi Jiedu granules by RP-HPLC

建立RP-HPLC法同时测定茵栀解毒颗粒中绿原酸和栀子苷的含量。采用反相高效液相色谱法,色谱柱为C_(18)柱(250.0 mm×4.6 mm,5μm),柱温30℃,流动相为乙腈‒甲醇‒0.1%磷酸(11∶8∶81),等度洗脱,检测波长为(235±1)nm,流速为1 mL/min。结果显示,绿原酸的线性范围为0.0943~0.7544μg,r=0.999999(n=5),平均回收率为99.25%(n=6);栀子苷线性范围为0.1971~1.5766μg,r=0.999999(n=5),平均回收率为99.56%(n=6)。该方法检测效率高、专属性强、重复性好,为茵栀解毒颗粒的质量评价提供了简便、准确、可靠的定量方法。

The objective was to establish a method for simultaneous determination of chlorogenic acid and geniposide in Yinzhi Jiedu granules by RP-HPLC.RP-HPLC method was developed.The separation was performed on a C_(18) column(250.0 mm×4.6 mm,5μm)with isocratic elution using acetomitrile-methanol-0.1%phosphate solution(11∶8∶81)as mobile phase,and column temperature was 30℃.The flow rate was 1 mL/min and the UV absorbance was monitored at(235±1)nm.The results showed that the linear range of chorogenic acid was 0.0943-0.7544μg,r=0.999999(n=5),the mean recovery was 99.25%(n=6);that of geniposide was 0.1971-1.5766μg,r=0.999999(n=5),and the mean recovery was 99.56%(n=6).The method can be used for quality control of Yinzhi Jiedu granules with simplicity,accuracy and reliability.