铁甲秋海棠花粉离体萌发研究

In vitro Germination of Begonia masoniana Pollen

为探究铁甲秋海棠Begonia masoniana花粉离体萌发适宜条件,采用液体培养法,通过单因素试验考察蔗糖、硼酸、PEG-4000、氯化钙浓度以及不同培养时间对花粉管生长的影响。结果表明,铁甲秋海棠花粉离体萌发的最适培养基为5%蔗糖+0.01%硼酸+5%PEG-4000+0.003%氯化钙;最佳观察时间为120 min。利用该培养基进行培养,铁甲秋海棠花粉萌发率达90.86%,花粉管长度达511.51μm。该研究结果可为同属其他种类秋海棠的花粉研究及育种提供参考。

To explore the suitable conditions for in vitro germination of Begonia masoniana pollen,a liquid culture method was used to investigate the effects of sucrose,boric acid,PEG-4000,calcium chloride concentration,and different cultivation times on pollen tube growth through single factor experiments.The results showed that the optimal medium for pollen germination was sucrose 5%,boric acid 0.01%,PEG-40005%,calcium chloride 0.003%.The optimal observation time was 120 min.The pollen germination rate of B.masoniana was 90.86%.The length of the pollen tube was 511.51μm.Our results could provide reference for pollen research and breeding of other begonias.