胸水细胞蜡块抗酸染色在诊断结核性胸膜炎中的应用研究

Study on the application of acid-fast staining of pleural effusion cell wax block in the diagnosis of tuberculous pleurisy

目的探讨胸水蜡块抗酸染色诊断结核性胸膜炎的价值。方法对77例结核性胸膜炎患者胸腔积液同时进行常规的胸水涂片检查和胸水离心沉淀物行石蜡切片,进行抗酸染色,统计抗酸染色阳性率。结果胸水常规涂片发现2例患者(2.6%)抗酸染色阳性;而胸水细胞蜡块有12例患者(15.58%)抗酸染色阳性。两者的抗酸染色阳性率统计分析得P=0.009375。结论胸水沉淀后制作细胞蜡块进行切片抗酸染色,可以显著提高结核分枝杆菌的检出率。能够为结核性胸膜炎的诊断提供病原学的依据,具有较高的诊断意义。

Objective To explore the value of acid fast staining of paraffin embedding of pleural effusion in the diagnosis of tuberculous pleurisy.Methods The pleural effusion of 77 patients with tuberculous pleurisy was examined by routine pleural smear and paraffin section of the precipitate after pleural centrifugation.The positive rate of acid fast staining was counted.Results Two patient(2.6%)showed positive acid fast staining in routine smear of pleural effusion;Twelve patients(15.58%)were positive for acid fast staining.The positive rate of acid fast staining was statistically analyzed(P=0.009375).Conclusion Pleural fluid paraffin embedded sections for acid fast staining after pleural precipitation can significantly improve the detection rate of Mycobacterium tuberculosis.It can provide etiological basis for the diagnosis of tuberculous pleurisy and has high diagnostic significance.